RNF70 (PJA1) (NM_145119) Human Untagged Clone

SKU SC124584

PJA1 (untagged)-Human praja ring finger 1 (PJA1), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: RNF70
• Synonyms: PRAJA1; RNF70
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC124584 sequence for NM_145119 edited (data generated by NextGen Sequencing); ATGGGTCAGGAATCTAGCAAGCCTGTATGGCCCAATCCAACAGGAGGGTATCAGTCCAAT; ACAGGTAGGAGGTATGGAAGAAGGCATGCTTATGTCAGTTTCAGGCCACCCACGAGCCAG; CGGGAAAGGATTGCCAGCCAGAGAAAGACGAACTCCGAAGTCCCAATGCACAGATCAGCC; CCCAGTCAAACCACCAAGAGGAGCCGATCGCCATTTTCCACTACTCGTCGTAGTTGGGAC; GACAGCGAGAGTTCGGGAACCAACCTGAATATTGATAATGAGGACTATTCCAGGTATCCG; CCAAGAGAGTACAGAGCTTCGGGTAGCAGAAGAGGAATGGCCTATGGACATATTGACTCT; TATGGGGCAGATGATAGTGAGGAGGAGGGGGCTGGGCCTGTTGAGCGACCGCCAGTGAGA; GGGAAAACTGGCAAGTTTAAAGATGATAAGCTGTATGACCCAGAGAAAGGGGCAAGGTCT; TTGGCTGGGCCACCTCCACATTTCTCTAGTTTTAGCCGTGATGTGAGAGAGGAGCGAGAC; AAGTTAGACCCAGTCCCTGCAGCAAGATGCTCAGCTAGCAGAGCTGACTTCCTGCCACAA; AGTAGTGTGGCCTCACAGTCGTCTTCTGAAGGCAAGCTGGCTACAAAAGGTGACAGCTCG; GAGAGGGAGAGAAGGGAGCAAAATTTACCTGCACGTCCCAGCAGGGCTCCTGTGAGTATT; TGTGGTGGTGGGGAAAACACCTCAAAGAGTGCAGAGGAACCTGTGGTCAGGCCCAAAATC; AGAAACCTGGCAAGTCCAAACTGCGTGAAACCAAAAATTTTTTTTGATACTGATGATGAT; GACGATATGCCACACAGTACTTCCAGGTGGAGGGATACCGCCAATGACAATGAGGGCCAC; TCGGATGGCCTGGCAAGAAGAGGGAGAGGCGAGAGTTCAAGTGGCTATCCCGAGCCAAAG; TACCCTGAAGACAAACGGGAAGCGAGGAGTGACCAAGTGAAACCAGAAAAGGTGCCGAGA; CGACGACGCACCATGGCCGACCCTGACTTCTGGACGCACAGTGATGATTACTACAAATAC; TGCGACGAAGACTCTGACAGTGACAAAGAGTGGATTGCTGCTCTGCGTCGGAAATATCGA; AGCCGAGAGCAAACCCTGTCCTCCAGTGGCGAAAGCTGGGAGACTCTGCCGGGGAAAGAA; GAGCGGGAACCTCCACAGGCTAAGGTGAGTGCCAGCACTGGCACCAGCCCTGGCCCCGGT; GCTAGTGCCAGTGCCGGGGCTGGCGCCGGGGCCAGTGCTGGCAGCAATGGCAGCAATTAC; CTTGAAGAAGTTCGAGAACCATCTCTTCAGGAAGAGCAGGCATCCCTGGAAGAAGGAGAA; ATTCCTTGGCTCCAGTACCATGAGAATGACAGTAGCAGTGAGGGGGATAATGATTCTGGT; CACGAGTTGATGCAACCTGGGGTATTCATGCTGGATGGAAACAACAACCTTGAAGATGAC; TCCAGTGTGAGCGAAGACCTAGAAGTGGATTGGAGCCTCTTTGATGGATTTGCAGATGGG; TTAGGAGTGGCTGAAGCCATTTCCTATGTGGACCCTCAGTTCCTCACCTACATGGCACTT; GAAGAACGCCTGGCCCAGGCAATGGAAACTGCCCTTGCGCACTTGGAGTCTCTCGCAGTG; GATGTAGAGGTGGCCAATCCACCAGCAAGCAAGGAGAGCATTGACGCTCTTCCCGAGATC; CTGGTCACTGAAGATCATGGCGCAGTTGGTCAGGAGATGTGCTGCCCCATCTGCTGTAGC; GAATATGTGAAGGGGGAGGTGGCAACTGAGCTGCCGTGCCACCACTATTTCCACAAGCCG; TGTGTGTCCATCTGGCTTCAGAAGTCAGGCACCTGCCCCGTGTGCCGCTGCATGTTCCCT; CCCCCACTCTAA; ; Clone variation with respect to NM_145119.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_145119 unedited; AATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCTCGTGCCGAATTCGGCA; CGAGGGGGAGCAGGAGCCCTCCTACCCGAGCCCCAGCTGTATTGGAAACCCTCCTAAGTC; AGAGTGATGGGTCAGGAATCTAGCAAGCCTGTATGGCCCAATCCAACAGGAGGGTATCAG; TCCAATACAGGTAGGAGGTATGGAAGAAGGCATGCTTATGTCAGTTTCAGGCCACCCACG; AGCCAGCGGGAAAGGATTGCCAGCCAGAGAAAGACGAACTCCGAAGTCCCAATGCACAGA; TCAGCCCCCAGTCAAACCACCAAGAGGAGCCGATCGCCATTTTCCACTACTCGTCGTAGT; TGGGACGACAGCGAGAGTTCGGGAACCAACCTGAATATTGATAATGAGGACTATTCCAGG; TATCCGCCAAGAGAGTACAGAGCTTCGGGTAGCAGAAGAGGAATGGCCTATGGACATATT; GACTCTTATGGGGCAGATGATAGTGAGGAGGAGGGGGCTGGGCCTGTTGAGCGACCGCCA; GTGAGAGGGAAAACTGGCAAGTTTAAAGATGATAAGCTGTATGACCCAGAGAAAGGGGCA; AGGTCTTTGGCTGGGCCACCTCCACATTTCTCTAGTTNTAACCGTGATGTGAGAGAGGAG; CGAGACAAGTTAAACCCAGTCCCTGCAGCAAGATGCTCAGCTAGCAGAGCTGACTTTCTG; CCACAAGTAGTGTGGCCTCACAGTCGTCTTCTGAAGCAAGCCTGCTACAAAAGTGACAGC; TCGNAGAGGGAGAAAGGGAGCAAATTTACCTGCCCTCCCACAGGCTCCTGTGGTATTGGG; TGGTTGGGAAACCCTCAAAATGCGAGGACCTGGGTCAGCCCAAATAGAACTGGCAGTCAA; CTGCTGAACCAAATTTTTTGTA;
• Restriction Sites: NotI-NotI
• ACCN: NM_145119
• Insert Size: 2550 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_145119.1, NP_660095.1
• RefSeq Size: 2700 bp
• RefSeq ORF: 1932 bp
• Locus ID: 64219
• UniProt ID: Q8NG27
• Cytogenetics: Xq13.1
• Protein Families: Druggable Genome
• Summary: This gene encodes an enzyme that has E2-dependent E3 ubiquitin-protein ligase activity. This enzyme belongs to a class of ubiquitin ligases that include a RING finger motif, and it can interact with the E2 ubiquitin-conjugating enzyme UbcH5B. This gene is located in an area of chromosome X where several X-linked cognitive disability disorders have been associated, and it has also been found as part of a contiguous gene deletion associated with craniofrontonasal syndrome, though a direct link to any disorder has yet to be demonstrated. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2010]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a).