Transitional B cell

Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-CD19 mouse monoclonal antibody. (UM500071; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD5 mouse monoclonal antibody. (Clone UMAB9, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-MME (CD10) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 3min, UM800127) (1:600)

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-MME (CD10) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 3min, UM800127) (1:600)

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD5 mouse monoclonal antibody. (Clone UMAB9, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-MME (CD10) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 3min, UM800128) (1:600)

Immunohistochemical staining of paraffin-embedded human tonsil using FCER2 (CD23) clone UMAB101, mouse monoclonal antibody at 1:400 dilution of 1mg/mL using Polink2 Broad HRP DAB for detection. UM800031 requires heat-induced epitope retrieval with citrate pH6.0 at 110°C for 3min using pressure chamber/cooker. The image is a composite of 3 tonsils which show strong membranous and cytoplasmic staining however each tonsil has a unique expression pattern in the inner and outer germinal center.

Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-CD19 mouse monoclonal antibody. (UM500071; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD5 mouse monoclonal antibody. (Clone UMAB9, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded human tonsil using FCER2 (CD23) clone UMAB101, mouse monoclonal antibody at 1:400 dilution of 1mg/mL using Polink2 Broad HRP DAB for detection. UM800031 requires heat-induced epitope retrieval with citrate pH6.0 at 110°C for 3min using pressure chamber/cooker. The image is a composite of 3 tonsils which show strong membranous and cytoplasmic staining however each tonsil has a unique expression pattern in the inner and outer germinal center.

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-CD38 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800153) (1:300)

Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-CD19 mouse monoclonal antibody. (UM500071; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-MME (CD10) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 3min, UM800128) (1:600)

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-CD38 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800153) (1:300)

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-MME (CD10) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 3min, UM800127) (1:600)

Immunohistochemical staining of paraffin-embedded human tonsil using FCER2 (CD23) clone UMAB101, mouse monoclonal antibody at 1:400 dilution of 1mg/mL using Polink2 Broad HRP DAB for detection. UM800031 requires heat-induced epitope retrieval with citrate pH6.0 at 110°C for 3min using pressure chamber/cooker. The image is a composite of 3 tonsils which show strong membranous and cytoplasmic staining however each tonsil has a unique expression pattern in the inner and outer germinal center.

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-CD38 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800153) (1:300)

H&E staining of CD19 overexpressed cytosection (TS402922), HEK293T cells were transfected with cDNA clone RC202922, 4um sections, 4x magnification

Purified recombinant protein CD19 was analyzed by SDS-PAGE gel and Coomossie Blue Staining.

Purified recombinant protein CD21 was analyzed by SDS-PAGE gel and Coomossie Blue Staining.

Immunohistology staining on FCER2 overexpressed cytosection TS404335P5 with rabbit anti DDK clone OTIR5G2 C/N TA592569 at 1:2000 dilution 20m RT. Antibody staining was achieved with HIER Citrate pH6 , Polink1 with DAB chromogen detection kit (D11-18). Positive stain shown with the brown chromogen present. HEK293T cells were transfected with cDNA clone RC204335, 5 micron sections, 40x magnification