pCas-Guide-GFP-CRISPRa gene activation vector

SKU GE100074

pCas-Guide-GFP-CRISPRa vector, All-in-one Vector for CRISPR/Cas9 Gene activation with tGFP reporter, dCas9-VP64 and gRNA cloning site.

Product Data

• Function: All-in-one CRISPRa SAM vector
• Features:
  • BamH I and BsmB I can be used for genomic target sequence cloning
  • gRNA under U6 promoter
  • gRNA is modified with MS2 RNA aptamer in the loops
  • CMV driven dCas9-VP64, which can activate gene expression
  • For robust gene activation, needs to work with pCRISPRa-Enhancer
  • CF3 primer can be used to sequence target sequence after cloning
  • Contains tGFP reporter gene under EF1a promoter
• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS.
• Storage: Please store at -20°C for long term storage.
• Stability: The dried and the suspended DNA are stable for one year from date of shipping when stored at -20°C.
• Shipping: Ambient