EFEMP1 (NM_004105) Human Recombinant Protein
Recombinant protein of human EGF-containing fibulin-like extracellular matrix protein 1 (EFEMP1), transcript variant 1Bulk Request/Custom Protein Development
Product Datasheet for 'TP314953'
|Description||Recombinant protein of human EGF-containing fibulin-like extracellular matrix protein 1 (EFEMP1), transcript variant 1|
|Expression cDNA Clone or AA Sequence||Recombinant protein was produced with TrueORF clone, RC214953. Click on the TrueORF clone link to view cDNA and protein sequences.|
|Predicted MW||52.7 kDa|
|Concentration||>50 ug/mL as determined by microplate BCA method|
|Purity||> 80% as determined by SDS-PAGE and Coomassie blue staining|
|Buffer||25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at –80C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.|
|Bioactivity||: 200 ng of recombinant purified EFEMP1-myc were added to cultured glioma cells (CNS-1 rat glioma cell line) for 6h, and cells were further processed for quantitative RT-PCR.
Results showed increased expression of metalloproteases MMP2 and MMP9, in agreement with published results from Hu. et. al. (Mol. Cancer Res. (2009) 7:1756-1770)
Data kindly provided by Dr. Mariano S. Viapiano, Ohio State University.
|Synonyms||DHRD; DRAD; FBLN3; FBNL; FLJ35535; MGC111353; MLVT; MTLV; S1-5;EGF-containing fibulin-like extracellular matrix protein 1; DRAD; FBLN3; FBNL; FLJ35535; MGC111353; MLVT; MTLV; S1-5; fibrillin-like; fibulin 3|
|Summary||This gene encodes a member of the fibulin family of extracellular matrix glycoproteins. Like all members of this family, the encoded protein contains tandemly repeated epidermal growth factor-like repeats followed by a C-terminus fibulin-type domain. This gene is upregulated in malignant gliomas and may play a role in the aggressive nature of these tumors. Mutations in this gene are associated with Doyne honeycomb retinal dystrophy. Alternatively spliced transcript variants that encode the same protein have been described.[provided by RefSeq, Nov 2009]|
|Protein Families||Secreted Protein|
|The use of this Proteins has been cited in the following citations:|
Novel paracrine modulation of Notch-DLL4 signaling by fibulin-3 promotes angiogenesis in high-grade gliomas
,Nandhu, MS;Hu, B;Cole, SE;Erdreich-Epstein, A;Rodriguez-Gil, DJ;Viapiano, MS;,
Cancer Res. Aug 2014
,PubMed ID 25139440