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EFEMP1 (NM_004105) Human Over-expression Lysate

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SKU
LY418212
Transient overexpression lysate of EGF-containing fibulin-like extracellular matrix protein 1 (EFEMP1), transcript variant 1 (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC214953
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_004105, NP_004096
Synonyms DHRD; DRAD; EGF-containing fibulin-like extracellular matrix protein 1; FBLN3; FBNL; fibrillin-like; fibulin 3; FLJ35535; MGC111353; MLVT; MTLV; S1-5
Predicted MW 54.6 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_004096
Locus ID 2202
Cytogenetics 2p16.1
Protein Families WB
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Other "EFEMP1" proteins (7)
SKU Description Size Price
PH304090 EFEMP1 MS Standard C13 and N15-labeled recombinant protein (NP_001034438) 10 ug
$3,360.00
PH314953 EFEMP1 MS Standard C13 and N15-labeled recombinant protein (NP_004096) 10 ug
$3,360.00
PH316753 EFEMP1 MS Standard C13 and N15-labeled recombinant protein (NP_001034437) 10 ug
$3,360.00
LC418212 Lyophilized EFEMP1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP304090 Recombinant protein of human EGF-containing fibulin-like extracellular matrix protein 1 (EFEMP1), transcript variant 3, 20 µg 20 ug
$737.00
TP314953 Recombinant protein of human EGF-containing fibulin-like extracellular matrix protein 1 (EFEMP1), transcript variant 1, 20 µg 20 ug
$737.00
TP316753 Recombinant protein of human EGF-containing fibulin-like extracellular matrix protein 1 (EFEMP1), transcript variant 2, 20 µg 20 ug
$737.00

file-alt Citations

The EFEMP1 Gene: A Frequent Target for Epigenetic Silencing in Multiple Human Pituitary Adenoma Subtypes
Duong, Yacqub-Usman, Emes et al
Neuroendocrinology (2013)
Cited by 15
AI Summary: OriGene products contributed to this study by providing the EFEMP1 cDNA clone (NM_004105) and the pTUNE inducible vector, which were used to generate the pT-EFEMP1 construct for enforced EFEMP1 expression experiments in GH3 cells.
Snippet: NM_004105), was purchased as a cDNA clone (OriGene) and was subcloned into pTUNE inducible vector (OriGene).The pTUNE vector, containing the complete coding sequence of EFEMP1 was designated, pT-EFEMP1.
Showing 1-1 of 1 papers.
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