CSK (NM_004383) Human Recombinant Protein
$737.00
In Stock*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-Myc/DDK |
| Predicted MW | 50.5 kDa |
| Concentration | >0.05 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Bioactivity | CSK activity verified in a biochemical assay: CSK (c-src tyrosine kinase) (TP310758) activity was measured in a homogeneous time-resolved fluorescent (HTRF®) assay. CSK is a tyrosine kinase known to phosphorylate LCK, FYN and LYN. Varying concentrations of CSK were added to a reaction mix containing ATP and a biotinylated kinase substrate and the reaction mixture was incubated to allow the protein to phosphorylate the tyrosine residue in the substrate. HTRF detection reagents were then added, and the time-resolved fluorescent signal was measured on a Flexstation 3 microplate reader. The time resolved fluorescent signal is expressed as “delta R” or “ΔR” and is a ratio calculated from the fluorescent emission intensities of the donor and acceptor fluors. |
| Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Dry Ice |
| Reference Data | |
| RefSeq | NP_004374 |
| Locus ID | 1445 |
| UniProt ID | P41240 |
| Cytogenetics | 15q24.1 |
| RefSeq Size | 2755 |
| RefSeq ORF | 1350 |
| Summary | The protein encoded by this gene is involved in multiple pathways, including the regulation of Src family kinases. It plays an important role in T-cell activation through its association with the protein encoded by the protein tyrosine phosphatase, non-receptor type 22 (PTPN22) gene. This protein also phosphorylates C-terminal tyrosine residues on multiple substrates, including the protein encoded by the SRC proto-oncogene, non-receptor tyrosine kinase gene. Phosphorylation suppresses the kinase activity of the Src family tyrosine kinases. An intronic polymorphism (rs34933034) in this gene has been found to affect B-cell activation and is associated with systemic lupus erythematosus (SLE). Alternative splicing results in multiple transcript variants. provided by RefSeq, Aug 2017 |
| Protein Categories | Enzyme: Kinases, Intracellular Proteins |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Chemokine signaling pathway, Epithelial cell signaling in Helicobacter pylori infection, Neurotrophin signaling pathway, Regulation of actin cytoskeleton |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "CSK" proteins (7)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH310758 | CSK MS Standard C13 and N15-labeled recombinant protein (NP_004374) | 10 ug |
$3,360.00
|
|
| PH325740 | CSK MS Standard C13 and N15-labeled recombinant protein (NP_001120662) | 10 ug |
$3,360.00
|
|
| LC401395 | Lyophilized CSK HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LC426695 | Lyophilized CSK HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LY401395 | Transient overexpression lysate of c-src tyrosine kinase (CSK), transcript variant 1 (as WB positive control) | 100 ug |
$436.00
|
|
| LY426695 | Transient overexpression lysate of c-src tyrosine kinase (CSK), transcript variant 2 (as WB positive control) | 100 ug |
$436.00
|
|
| TP325740 | Recombinant protein of human c-src tyrosine kinase (CSK), transcript variant 2, 20 µg | 20 ug |
$737.00
|
|
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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