E2F1 (NM_005225) Human Recombinant Protein
$867.00
In Stock*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-Myc/DDK |
| Predicted MW | 46.7 kDa |
| Concentration | >0.05 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Bioactivity | WB standard (PMID: 26639898) |
| Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Dry Ice |
| Reference Data | |
| RefSeq | NP_005216 |
| Locus ID | 1869 |
| UniProt ID | Q01094 |
| Cytogenetics | 20q11.22 |
| RefSeq Size | 2486 |
| RefSeq ORF | 1311 |
| Synonyms | E2F-1; RBAP1; RBBP3; RBP3 |
| Summary | The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. provided by RefSeq, Jul 2008 |
| Protein Categories | Growth Factors, Intracellular Proteins, Transciption Factors |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | Bladder cancer, Cell cycle, Chronic myeloid leukemia, Glioma, Melanoma, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer, Small cell lung cancer |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "E2F1" proteins (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH308247 | E2F1 MS Standard C13 and N15-labeled recombinant protein (NP_005216) | 10 ug |
$3,360.00
|
|
| LC417432 | Lyophilized E2F1 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LY417432 | Transient overexpression lysate of E2F transcription factor 1 (E2F1) (as WB positive control) | 100 ug |
$436.00
|
|
Citations
Recruitment of Pontin/Reptin by E2f1 amplifies E2f transcriptional response during cancer progression
Tarangelo, Lo, Teng et al
Nat Commun (2015) 6, 10028 DOI: 10.1038/ncomms10028
Tarangelo, Lo, Teng et al
Nat Commun (2015) 6, 10028 DOI: 10.1038/ncomms10028
Cited by 67
AI Summary: In this paper, OriGene products—specifically the pCMV6-E2f1 expression plasmid and E2f1 shRNA lentiviral particles—were used to modulate E2f1 levels in cells, enabling the authors to investigate how E2f1 recruitment of the Pontin/Reptin complex amplifies E2f transcriptional responses during cancer progression.
Snippet: Cite SaveSaving
Showing 1-1 of 1 papers.
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