OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

E2F1 (NM_005225) Human Over-expression Lysate

Be the first to review this product
SKU
LY417432
Transient overexpression lysate of E2F transcription factor 1 (E2F1) (as WB positive control)
  $436.00
In Stock*
Bulk/Customize

Frequently Bought Together (3)

TA590609 Rabbit Polyclonal E2F1 Antibody
100 ug
$482.00
TA811000S beta Actin Mouse Monoclonal Antibody, Clone OTI1, Loading Control 30 ul
$200.00
TA592569 DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
100 ul
$519.00

Other products for "E2F1 or NM_005225"

Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC208247
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_005225, NP_005216
Synonyms E2F-1; RBAP1; RBBP3; RBP3
Predicted MW 46.7 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_005216
Locus ID 1869
Cytogenetics 20q11.22
Protein Families WB
Protein Pathways Bladder cancer, Cell cycle, Chronic myeloid leukemia, Glioma, Melanoma, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer, Small cell lung cancer
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
Other "E2F1" proteins (3)
SKU Description Size Price
PH308247 E2F1 MS Standard C13 and N15-labeled recombinant protein (NP_005216) 10 ug
$3,360.00
LC417432 Lyophilized E2F1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP308247 Recombinant protein of human E2F transcription factor 1 (E2F1), 20 µg 20 ug
$867.00

file-alt Citations

Non-overlapping Control of Transcriptome by Promoter- and Super-Enhancer-Associated Dependencies in Multiple Myeloma
Fulciniti, Lin, Samur et al
Cell Rep (2018) 25 (13), 3693-3705.e6 DOI: 10.1016/j.celrep.2018.12.016
AI Summary: OriGene’s E2F1 (NM_005225) Human Tagged ORF Clone (RC208247L2) was used in this study to investigate the role of E2F1 in multiple myeloma, enabling functional analysis of E2F1’s contribution to distinct transcriptional regulatory programs.
Snippet: ;TRIPZ Human E2F1 shRNA V2THS_43498 Thermo Scientific Bio RHS4696-200701670 E2F1 (NM_005225) Human Tagged ORF Clone Origene RC208247L2
CDC20B is required for deuterosome-mediated centriole production in multiciliated cells
Revinski, Zaragosi, Boutin et al
Nat Commun (2018) 9 (1), 4668 DOI: 10.1038/s41467-018-06768-z
Cited by 87
AI Summary: OriGene products contributed to this study by providing the CDC20B Myc-DDK tagged expression plasmid (Cat# RC223434), which was used for CDC20B overexpression experiments to investigate its role in centriole production in multiciliated cells.
FigureFigureFigure
Showing 1-2 of 2 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products