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E2F1 (NM_005225) Human Over-expression Lysate

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SKU
LC417432
Lyophilized E2F1 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC208247
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_005225, NP_005216
Synonyms E2F-1; RBAP1; RBBP3; RBP3
Predicted MW 46.7 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_005216
Locus ID 1869
Cytogenetics 20q11.22
Protein Families WB
Protein Pathways Bladder cancer, Cell cycle, Chronic myeloid leukemia, Glioma, Melanoma, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer, Small cell lung cancer
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Other "E2F1" proteins (3)
SKU Description Size Price
PH308247 E2F1 MS Standard C13 and N15-labeled recombinant protein (NP_005216) 10 ug
$3,360.00
LY417432 Transient overexpression lysate of E2F transcription factor 1 (E2F1) (as WB positive control) 100 ug
$436.00
TP308247 Recombinant protein of human E2F transcription factor 1 (E2F1), 20 µg 20 ug
$867.00

file-alt Citations

Non-overlapping Control of Transcriptome by Promoter- and Super-Enhancer-Associated Dependencies in Multiple Myeloma
Fulciniti, Lin, Samur et al
Cell Rep (2018) 25 (13), 3693-3705.e6 DOI: 10.1016/j.celrep.2018.12.016
AI Summary: OriGene’s E2F1 (NM_005225) Human Tagged ORF Clone (RC208247L2) was used in this study to investigate the role of E2F1 in multiple myeloma, enabling functional analysis of E2F1’s contribution to distinct transcriptional regulatory programs.
Snippet: ;TRIPZ Human E2F1 shRNA V2THS_43498 Thermo Scientific Bio RHS4696-200701670 E2F1 (NM_005225) Human Tagged ORF Clone Origene RC208247L2
CDC20B is required for deuterosome-mediated centriole production in multiciliated cells
Revinski, Zaragosi, Boutin et al
Nat Commun (2018) 9 (1), 4668 DOI: 10.1038/s41467-018-06768-z
Cited by 87
AI Summary: OriGene products contributed to this study by providing the CDC20B Myc-DDK tagged expression plasmid (Cat# RC223434), which was used for CDC20B overexpression experiments to investigate its role in centriole production in multiciliated cells.
FigureFigureFigure
Showing 1-2 of 2 papers.
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