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EIF4E (NM_001968) Human Recombinant Protein

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SKU
TP307333
Recombinant protein of human eukaryotic translation initiation factor 4E (EIF4E), transcript variant 1, 20 µg
Size:
20 ug 1 mg 100 ug
  $737.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293
Expression cDNA Clone or AA Sequence
Protein Sequence
>RC207333 protein sequence
Red=Cloning site Green=Tags(s)

MATVEPETTPTPNPPTTEEEKTESNQEVANPEHYIKHPLQNRWALWFFKNDKSKTWQANLRLISKFDTVE
DFWALYNHIQLSSNLMPGCDYSLFKDGIEPMWEDEKNKRGGRWLITLNKQQRRSDLDRFWLETLLCLIGE
SFDDYSDDVCGAVVNVRAKGDKIAIWTTECENREAVTHIGRVYKERLGLPPKIVIGYQSHADTATKSGST
TKNRFVV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag C-Myc/DDK
Predicted MW 24.9 kDa
Concentration >0.05 µg/µL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Preparation Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping Dry Ice
Reference Data
RefSeq NP_001959
Locus ID 1977
UniProt ID P06730
Cytogenetics 4q23
RefSeq Size 4749
RefSeq ORF 651
Synonyms AUTS19; CBP; eIF-4E; EIF4E1; EIF4EL1; EIF4F
Summary The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants. provided by RefSeq, Sep 2015
Protein Categories Intracellular Proteins
Protein Pathways Insulin signaling pathway, mTOR signaling pathway
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Other "EIF4E" proteins (4)
SKU Description Size Price
PH307333 EIF4E MS Standard C13 and N15-labeled recombinant protein (NP_001959) 10 ug
$3,360.00
LC400723 Lyophilized EIF4E HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY400723 Transient overexpression lysate of eukaryotic translation initiation factor 4E (EIF4E), transcript variant 1 (as WB positive control) 100 ug
$436.00
TP721168 Purified recombinant protein of Human eukaryotic translation initiation factor 4E (EIF4E), transcript variant 1 10 ug
$340.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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