EIF4E (NM_001968) Human Recombinant Protein
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | E. coli |
| Expression cDNA Clone or AA Sequence | |
| Tag | Tag Free |
| Predicted MW | 25.1 kDa |
| Concentration | lot specific |
| Purity | >95% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | Lyophilized from a 0.2 um filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
| Endotoxin | Endotoxin level is < 0.1 ng/µg of protein (< 1 EU/µg) |
| Reconstitution Method | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. Dissolve the lyophilized protein in ddH2O. It is not recommended to reconstitute a concentration less than 100 µg/ml. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Storage | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
| Stability | Stable for at least 6 months from date of receipt under proper storage and handling conditions. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NP_001959 |
| Locus ID | 1977 |
| UniProt ID | P06730 |
| Cytogenetics | 4q23 |
| RefSeq Size | 4749 |
| RefSeq ORF | 651 |
| Synonyms | AUTS19; CBP; eIF-4E; EIF4E1; EIF4EL1; EIF4F |
| Summary | The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants. provided by RefSeq, Sep 2015 |
| Protein Categories | Intracellular Proteins |
| Protein Pathways | Insulin signaling pathway, mTOR signaling pathway |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "EIF4E" proteins (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH307333 | EIF4E MS Standard C13 and N15-labeled recombinant protein (NP_001959) | 10 ug |
$3,360.00
|
|
| LC400723 | Lyophilized EIF4E HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LY400723 | Transient overexpression lysate of eukaryotic translation initiation factor 4E (EIF4E), transcript variant 1 (as WB positive control) | 100 ug |
$436.00
|
|
| TP307333 | Recombinant protein of human eukaryotic translation initiation factor 4E (EIF4E), transcript variant 1, 20 µg | 20 ug |
$737.00
|
|
Citations
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