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MGMT (NM_002412) Human Over-expression Lysate

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SKU
LY432155
Transient overexpression lysate of O-6-methylguanine-DNA methyltransferase (MGMT) (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC229131
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_002412, NP_002403
Predicted MW 24.9 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_002403
Locus ID 4255
Cytogenetics 10q26.3
Protein Families WB
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Other "MGMT" proteins (4)
SKU Description Size Price
LC432155 Lyophilized MGMT HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP329131 Recombinant protein of human O-6-methylguanine-DNA methyltransferase (MGMT), 20 µg 20 ug
$737.00
TP721020 Purified recombinant protein of Human O-6-methylguanine-DNA methyltransferase (MGMT) 10 ug
$260.00
TP760054 Recombinant protein of human O-6-methylguanine-DNA methyltransferase (MGMT), full length, with N-terminal HIS tag, expressed in E.Coli, 50ug 50 ug
$270.00

file-alt Citations

ATR inhibition reverses the resistance of homologous recombination deficient MGMTlow/MMRproficient cancer cells to temozolomide
El Touny, Hose, Connelly et al
Oncotarget (2021) 12 (21), 2114-2130 DOI: 10.18632/oncotarget.28090
Cited by 12
Snippet: cytometer/CellQuest Pro. MGMT overexpression vector cloning The human MGMT cDNA sequence was amplified using Phusion High-Fidelity DNA Polymerase (NEB) from the Myc-DDK-tagged MGMT cDNA template (Origene, NM_002412) and cloned into the lentiviral vector pCDH-pGK-puro using BamHI/EcoRI sites. MGMT overexpression and RAD51 knockdown Target cells were spinoculated at a density of 2 × 105/well i
FigureFigureFigure
Showing 1-1 of 1 papers.
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