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MGMT (NM_002412) Human Over-expression Lysate

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SKU
LC432155
Lyophilized MGMT HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC229131
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_002412, NP_002403
Predicted MW 24.9 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_002403
Locus ID 4255
Cytogenetics 10q26.3
Protein Families WB
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Other "MGMT" proteins (4)
SKU Description Size Price
LY432155 Transient overexpression lysate of O-6-methylguanine-DNA methyltransferase (MGMT) (as WB positive control) 100 ug
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$737.00
TP721020 Purified recombinant protein of Human O-6-methylguanine-DNA methyltransferase (MGMT) 10 ug
$260.00
TP760054 Recombinant protein of human O-6-methylguanine-DNA methyltransferase (MGMT), full length, with N-terminal HIS tag, expressed in E.Coli, 50ug 50 ug
$270.00

file-alt Citations

ATR inhibition reverses the resistance of homologous recombination deficient MGMTlow/MMRproficient cancer cells to temozolomide
El Touny, Hose, Connelly et al
Oncotarget (2021) 12 (21), 2114-2130 DOI: 10.18632/oncotarget.28090
Cited by 12
Snippet: cytometer/CellQuest Pro. MGMT overexpression vector cloning The human MGMT cDNA sequence was amplified using Phusion High-Fidelity DNA Polymerase (NEB) from the Myc-DDK-tagged MGMT cDNA template (Origene, NM_002412) and cloned into the lentiviral vector pCDH-pGK-puro using BamHI/EcoRI sites. MGMT overexpression and RAD51 knockdown Target cells were spinoculated at a density of 2 × 105/well i
FigureFigureFigure
Showing 1-1 of 1 papers.
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