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PRKAR1B (NM_001164759) Human Over-expression Lysate

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SKU
LY431888
Transient overexpression lysate of protein kinase, cAMP-dependent, regulatory, type I, beta (PRKAR1B), transcript variant 4 (as WB positive control)
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC228860
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001164759, NP_001158231
Synonyms PRKAR1
Predicted MW 43.1 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_001158231
Locus ID 5575
Cytogenetics 7p22.3
Protein Families WB
Protein Pathways Apoptosis, Insulin signaling pathway
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Other "PRKAR1B" proteins (18)
SKU Description Size Price
PH307809 PRKAR1B MS Standard C13 and N15-labeled recombinant protein (NP_002726) 10 ug
$3,360.00
LC400964 Lyophilized PRKAR1B HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC431887 Lyophilized PRKAR1B HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC431888 Lyophilized PRKAR1B HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC431889 Lyophilized PRKAR1B HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC431890 Lyophilized PRKAR1B HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC431891 Lyophilized PRKAR1B HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY400964 Transient overexpression lysate of protein kinase, cAMP-dependent, regulatory, type I, beta (PRKAR1B), transcript variant 2 (as WB positive control) 100 ug
$436.00
LY431887 Transient overexpression lysate of protein kinase, cAMP-dependent, regulatory, type I, beta (PRKAR1B), transcript variant 3 (as WB positive control) 100 ug
$436.00
LY431889 Transient overexpression lysate of protein kinase, cAMP-dependent, regulatory, type I, beta (PRKAR1B), transcript variant 5 (as WB positive control) 100 ug
$436.00
LY431890 Transient overexpression lysate of protein kinase, cAMP-dependent, regulatory, type I, beta (PRKAR1B), transcript variant 6 (as WB positive control) 100 ug
$436.00
LY431891 Transient overexpression lysate of protein kinase, cAMP-dependent, regulatory, type I, beta (PRKAR1B), transcript variant 1 (as WB positive control) 100 ug
$436.00
TP307809 Recombinant protein of human protein kinase, cAMP-dependent, regulatory, type I, beta (PRKAR1B), 20 µg 20 ug
$867.00
TP328859 Purified recombinant protein of Homo sapiens protein kinase, cAMP-dependent, regulatory, type I, beta (PRKAR1B), transcript variant 3, 20 µg 20 ug
$867.00
TP328860 Purified recombinant protein of Homo sapiens protein kinase, cAMP-dependent, regulatory, type I, beta (PRKAR1B), transcript variant 4, 20 µg 20 ug
$867.00
TP328861 Purified recombinant protein of Homo sapiens protein kinase, cAMP-dependent, regulatory, type I, beta (PRKAR1B), transcript variant 5, 20 µg 20 ug
$867.00
TP328862 Purified recombinant protein of Homo sapiens protein kinase, cAMP-dependent, regulatory, type I, beta (PRKAR1B), transcript variant 6, 20 µg 20 ug
$867.00
TP328863 Purified recombinant protein of Homo sapiens protein kinase, cAMP-dependent, regulatory, type I, beta (PRKAR1B), transcript variant 1, 20 µg 20 ug
$867.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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