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PCNA (NM_182649) Human Over-expression Lysate

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SKU
LY430596
Transient overexpression lysate of proliferating cell nuclear antigen (PCNA), transcript variant 2 (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC209379
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_182649, NP_872590
Synonyms ATLD2
Predicted MW 28.8 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_872590
Locus ID 5111
Cytogenetics 20p12.3
Protein Families WB
Protein Pathways Base excision repair, Cell cycle, DNA replication, Mismatch repair, Nucleotide excision repair
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Other "PCNA" proteins (9)
SKU Description Size Price
PH301741 PCNA MS Standard C13 and N15-labeled recombinant protein (NP_002583) 10 ug
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LC400929 Lyophilized PCNA HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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LC405433 Lyophilized PCNA HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC430596 Lyophilized PCNA HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY400929 Transient overexpression lysate of proliferating cell nuclear antigen (PCNA), transcript variant 1 (as WB positive control) 100 ug
$436.00
LY405433 Transient overexpression lysate of proliferating cell nuclear antigen (PCNA), transcript variant 2 (as WB positive control) 100 ug
$436.00
TP301741 Recombinant protein of human proliferating cell nuclear antigen (PCNA), transcript variant 1, 20 µg 20 ug
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TP750150 Purified recombinant protein of Human proliferating cell nuclear antigen (PCNA), transcript variant 2, full length, tag free, expressed in E.Coli, 50 ug 50 ug
$270.00
TP760481 Purified recombinant protein of Human proliferating cell nuclear antigen (PCNA), transcript variant 2, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug 50 ug
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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