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BAD (NM_004322) Human Over-expression Lysate

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SKU
LY429193
Transient overexpression lysate of BCL2-associated agonist of cell death (BAD), transcript variant 1 (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC200634
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_004322, NP_004313
Synonyms BBC2; BCL2L8
Predicted MW 18.2 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_004313
Locus ID 572
Cytogenetics 11q13.1
Protein Families WB
Protein Pathways Acute myeloid leukemia, Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Focal adhesion, Insulin signaling pathway, Melanoma, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer, VEGF signaling pathway
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Other "BAD" proteins (8)
SKU Description Size Price
PH300634 BAD MS Standard C13 and N15-labeled recombinant protein (NP_004313) 10 ug
$3,360.00
LC403214 Lyophilized BAD HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC418067 Lyophilized BAD HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC429193 Lyophilized BAD HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY403214 Transient overexpression lysate of BCL2-associated agonist of cell death (BAD), transcript variant 2 (as WB positive control) 100 ug
$436.00
LY418067 Transient overexpression lysate of BCL2-associated agonist of cell death (BAD), transcript variant 1 (as WB positive control) 100 ug
$436.00
TP300634 Recombinant protein of human BCL2-associated agonist of cell death (BAD), transcript variant 1, 20 µg 20 ug
$737.00
TP760222 Recombinant protein of human BCL2-associated agonist of cell death (BAD), transcript variant 2, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug 50 ug
$270.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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