NR2F2 (NM_001145155) Human Over-expression Lysate
SKU
LY428730
Transient overexpression lysate of nuclear receptor subfamily 2, group F, member 2 (NR2F2), transcript variant 2 (as WB positive control)
$436.00
In Stock*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC226609 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_001145155, NP_001138627 |
| Synonyms | ARP-1; ARP1; CHTD4; COUPTF2; COUPTFB; COUPTFII; NF-E3; SRXX5; SVP40; TFCOUP2 |
| Predicted MW | 31.3 kDa |
| Components | 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer |
| Storage | The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. |
| Shipping | Dry Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). |
| Reference Data | |
| RefSeq | NP_001138627 |
| Locus ID | 7026 |
| Cytogenetics | 15q26.2 |
| Protein Families | WB |
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Resources
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Other "NR2F2" proteins (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| LC412132 | Lyophilized NR2F2 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LC428730 | Lyophilized NR2F2 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LY412132 | Transient overexpression lysate of nuclear receptor subfamily 2, group F, member 2 (NR2F2), transcript variant 1 (as WB positive control) | 100 ug |
$436.00
|
|
Citations
Venous Endothelial Marker COUP-TFII Regulates the Distinct Pathologic Potentials of Adult Arteries and Veins
Cui, Lu, Lee et al
Sci Rep (2015) 5, 16193 DOI: 10.1038/srep16193
Cui, Lu, Lee et al
Sci Rep (2015) 5, 16193 DOI: 10.1038/srep16193
Cited by 61
AI Summary: OriGene’s pCMV6-XL5-Nr2f2 plasmid was used as the source of human COUP-TFII cDNA for lentiviral overexpression experiments, enabling the study of COUP-TFII’s role in regulating vascular endothelial cell phenotypes in this paper.
Snippet: Lentivirus to Over-express COUP-TFII. The human Nr2f2 (COUP-TFII) cDNA was amplified from pCMV6-XL5-Nr2f2 (OriGene) by PCR, then sub-cloned into pCDH-EF1-T2A-GFP lentiviral vector (System Biosciences) between EcoRI and NotI digestion sites. ... Cite SaveSaving
Showing 1-1 of 1 papers.
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