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NR2F2 (NM_001145155) Human Over-expression Lysate

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SKU
LC428730
Lyophilized NR2F2 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC226609
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001145155, NP_001138627
Synonyms ARP-1; ARP1; CHTD4; COUPTF2; COUPTFB; COUPTFII; NF-E3; SRXX5; SVP40; TFCOUP2
Predicted MW 31.3 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_001138627
Locus ID 7026
Cytogenetics 15q26.2
Protein Families WB
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Other "NR2F2" proteins (3)
SKU Description Size Price
LC412132 Lyophilized NR2F2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY412132 Transient overexpression lysate of nuclear receptor subfamily 2, group F, member 2 (NR2F2), transcript variant 1 (as WB positive control) 100 ug
$436.00
LY428730 Transient overexpression lysate of nuclear receptor subfamily 2, group F, member 2 (NR2F2), transcript variant 2 (as WB positive control) 100 ug
$436.00

file-alt Citations

Venous Endothelial Marker COUP-TFII Regulates the Distinct Pathologic Potentials of Adult Arteries and Veins
Cui, Lu, Lee et al
Sci Rep (2015) 5, 16193 DOI: 10.1038/srep16193
Cited by 61
AI Summary: OriGene’s pCMV6-XL5-Nr2f2 plasmid was used as the source of human COUP-TFII cDNA for lentiviral overexpression experiments, enabling the study of COUP-TFII’s role in regulating vascular endothelial cell phenotypes in this paper.
Snippet: Lentivirus to Over-express COUP-TFII. The human Nr2f2 (COUP-TFII) cDNA was amplified from pCMV6-XL5-Nr2f2 (OriGene) by PCR, then sub-cloned into pCDH-EF1-T2A-GFP lentiviral vector (System Biosciences) between EcoRI and NotI digestion sites. ... Cite SaveSaving
FigureFigureFigure
Showing 1-1 of 1 papers.
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