OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Amyloid Precursor Protein (APP) (NM_001136129) Human Over-expression Lysate

Be the first to review this product
SKU
LY427815
Transient overexpression lysate of amyloid beta (A4) precursor protein (APP), transcript variant 5 (as WB positive control)
  $436.00
In Stock*
Bulk/Customize

Frequently Bought Together (3)

TA500969 Anti-APP (Amyloid beta precursor protein) mouse monoclonal antibody, clone OTI7G9 (formerly 7G9) 100 ul
$447.00
TA811000S beta Actin Mouse Monoclonal Antibody, Clone OTI1, Loading Control 30 ul
$200.00
TA592569 DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
100 ul
$519.00

Other products for "Amyloid Precursor Protein or NM_001136129"

Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC226848
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001136129, NP_001129601
Synonyms AAA; ABETA; ABPP; AD1; alpha-sAPP; APPI; CTFgamma; CVAP; PN-II; PN2; preA4
Predicted MW 72.55 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_001129601
Locus ID 351
Cytogenetics 21q21.3
Protein Families WB
Protein Pathways Alzheimer's disease
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
Other "Amyloid Precursor Protein" proteins (11)
SKU Description Size Price
PH309575 APP MS Standard C13 and N15-labeled recombinant protein (NP_958816) 10 ug
$3,360.00
PH315147 APP MS Standard C13 and N15-labeled recombinant protein (NP_958817) 10 ug
$3,360.00
LC404408 Lyophilized APP HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC404409 Lyophilized APP HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC424694 Lyophilized APP HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC427815 Lyophilized APP HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY404408 Transient overexpression lysate of amyloid beta (A4) precursor protein (APP), transcript variant 2 (as WB positive control) 100 ug
$436.00
LY404409 Transient overexpression lysate of amyloid beta (A4) precursor protein (APP), transcript variant 3 (as WB positive control) 100 ug
$665.00
LY424694 Transient overexpression lysate of amyloid beta (A4) precursor protein (APP), transcript variant 1 (as WB positive control) 100 ug
$665.00
TP309575 Recombinant protein of human amyloid beta (A4) precursor protein (APP), transcript variant 2, 20 µg 20 ug
$867.00
TP315147 Recombinant protein of human amyloid beta (A4) precursor protein (APP), transcript variant 3, 20 µg 20 ug
$867.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products