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BTK (NM_000061) Human Over-expression Lysate

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SKU
LY424947
Transient overexpression lysate of Bruton agammaglobulinemia tyrosine kinase (BTK) (as WB positive control)
  $665.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC211582
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_000061, NP_000052
Synonyms AGMX1; AT; ATK; BPK; IGHD3; IMD1; PSCTK1; XLA
Predicted MW 76.1 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_000052
Locus ID 695
Cytogenetics Xq22.1
Protein Families WB
Protein Pathways B cell receptor signaling pathway, Fc epsilon RI signaling pathway, Primary immunodeficiency
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Other "BTK" proteins (4)
SKU Description Size Price
PH311582 BTK MS Standard C13 and N15-labeled recombinant protein (NP_000052) 10 ug
$3,360.00
LC424947 Lyophilized BTK HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP311582 Recombinant protein of human Bruton agammaglobulinemia tyrosine kinase (BTK), 20 µg 20 ug
$737.00
TP710016 Recombinant protein of human Bruton agammaglobulinemia tyrosine kinase (BTK),full length, with C-terminal polyhistidine tag, expressed in sf9 cell 20 ug
$540.00

file-alt Citations

X-Linked Agammaglobulinemia Associated with B-Precursor Acute Lymphoblastic Leukemia
Hoshino, Okuno, Migita et al
J. Clin. Immunol. (2015)
Cited by 25
AI Summary: In this study, OriGene's BTK antibody (clone 10E10) was used to assess BTK protein expression levels in patient samples, helping to demonstrate reduced BTK expression associated with X-linked agammaglobulinemia and B-precursor acute lymphoblastic leukemia.
Snippet: Black histograms indicate BTK expression (clone 10E10, OriGene Technologies, Inc., Rockville, MD), and Gray histograms indicate isotype control. BTK expression in the patient was reduced compared with that in a normal control. ... Related articles All 3 versions Cite SaveSaving
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