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BTK (NM_000061) Human Over-expression Lysate

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SKU
LC424947
Lyophilized BTK HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC211582
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_000061, NP_000052
Synonyms AGMX1; AT; ATK; BPK; IGHD3; IMD1; PSCTK1; XLA
Predicted MW 76.1 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_000052
Locus ID 695
Cytogenetics Xq22.1
Protein Families WB
Protein Pathways B cell receptor signaling pathway, Fc epsilon RI signaling pathway, Primary immunodeficiency
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Other "BTK" proteins (4)
SKU Description Size Price
PH311582 BTK MS Standard C13 and N15-labeled recombinant protein (NP_000052) 10 ug
$3,360.00
LY424947 Transient overexpression lysate of Bruton agammaglobulinemia tyrosine kinase (BTK) (as WB positive control) 100 ug
$665.00
TP311582 Recombinant protein of human Bruton agammaglobulinemia tyrosine kinase (BTK), 20 µg 20 ug
$737.00
TP710016 Recombinant protein of human Bruton agammaglobulinemia tyrosine kinase (BTK),full length, with C-terminal polyhistidine tag, expressed in sf9 cell 20 ug
$540.00

file-alt Citations

X-Linked Agammaglobulinemia Associated with B-Precursor Acute Lymphoblastic Leukemia
Hoshino, Okuno, Migita et al
J. Clin. Immunol. (2015)
Cited by 25
AI Summary: In this study, OriGene's BTK antibody (clone 10E10) was used to assess BTK protein expression levels in patient samples, helping to demonstrate reduced BTK expression associated with X-linked agammaglobulinemia and B-precursor acute lymphoblastic leukemia.
Snippet: Black histograms indicate BTK expression (clone 10E10, OriGene Technologies, Inc., Rockville, MD), and Gray histograms indicate isotype control. BTK expression in the patient was reduced compared with that in a normal control. ... Related articles All 3 versions Cite SaveSaving
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Showing 1-1 of 1 papers.
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