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PTCH1 (NM_000264) Human Over-expression Lysate

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SKU
LY424834
Transient overexpression lysate of patched homolog 1 (Drosophila) (PTCH1), transcript variant 1b (as WB positive control)
  $665.00
4 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC216999
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_000264, NP_000255
Synonyms BCNS; NBCCS; PTC; PTC1; PTCH
Predicted MW 160.4 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_000255
Locus ID 5727
Cytogenetics 9q22.32
Protein Families WB
Protein Pathways Basal cell carcinoma, Hedgehog signaling pathway, Pathways in cancer
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Other "PTCH1" proteins (3)
SKU Description Size Price
LC424834 Lyophilized PTCH1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP710156 Recombinant protein of human patched homolog 1 (Drosophila) (PTCH1), transcript variant 1b, residues 770-1027, with C-terminal DDK tag, expressed in sf9, 20ug 20 ug
$540.00
TP762274 Purified recombinant protein of Human patched 1 (PTCH1), transcript variant 1b, Arg770-Trp1027, with N-terminal His tag, expressed in E.coli, 50ug 50 ug
$260.00

file-alt Citations

Hedgehog signaling in the airway epithelium of patients with chronic obstructive pulmonary disease
Tam, Hughes, McNagny et al
Sci Rep (2019) 9 (1), 3353 DOI: 10.1038/s41598-019-40045-3
AI Summary: OriGene’s HEK293T cell lysate overexpressing human PTCH1 (catalog number LY424834) was used as a positive control to confirm the specificity of the PTCH1 antibody in this study on Hedgehog signaling in COPD airway epithelium.
Snippet: nti-Rabbit IgG (A-11001, ThermoFisher) for 2 h at room temperature, and visualized using LI-COR Odyssey CLx (LICOR, Lincoln, Nebraska USA). HEK293T cell lysate overexpressing human PTCH1 (LY424834, Origene) as positive control and human airway epithelial cells were used to confirm PTCH1 antibody specificity. Immunofluorescence microscopy on differentiated cell culture Apical ALI culture compart
FigureFigureFigure
Showing 1-1 of 1 papers.
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