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PTCH1 (NM_000264) Human Over-expression Lysate

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SKU
LC424834
Lyophilized PTCH1 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC216999
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_000264, NP_000255
Synonyms BCNS; NBCCS; PTC; PTC1; PTCH
Predicted MW 160.4 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_000255
Locus ID 5727
Cytogenetics 9q22.32
Protein Families WB
Protein Pathways Basal cell carcinoma, Hedgehog signaling pathway, Pathways in cancer
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Other "PTCH1" proteins (3)
SKU Description Size Price
LY424834 Transient overexpression lysate of patched homolog 1 (Drosophila) (PTCH1), transcript variant 1b (as WB positive control) 100 ug
$665.00
TP710156 Recombinant protein of human patched homolog 1 (Drosophila) (PTCH1), transcript variant 1b, residues 770-1027, with C-terminal DDK tag, expressed in sf9, 20ug 20 ug
$540.00
TP762274 Purified recombinant protein of Human patched 1 (PTCH1), transcript variant 1b, Arg770-Trp1027, with N-terminal His tag, expressed in E.coli, 50ug 50 ug
$260.00

file-alt Citations

Hedgehog signaling in the airway epithelium of patients with chronic obstructive pulmonary disease
Tam, Hughes, McNagny et al
Sci Rep (2019) 9 (1), 3353 DOI: 10.1038/s41598-019-40045-3
AI Summary: OriGene’s HEK293T cell lysate overexpressing human PTCH1 (catalog number LY424834) was used as a positive control to confirm the specificity of the PTCH1 antibody in this study on Hedgehog signaling in COPD airway epithelium.
Snippet: nti-Rabbit IgG (A-11001, ThermoFisher) for 2 h at room temperature, and visualized using LI-COR Odyssey CLx (LICOR, Lincoln, Nebraska USA). HEK293T cell lysate overexpressing human PTCH1 (LY424834, Origene) as positive control and human airway epithelial cells were used to confirm PTCH1 antibody specificity. Immunofluorescence microscopy on differentiated cell culture Apical ALI culture compart
FigureFigureFigure
Showing 1-1 of 1 papers.
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