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Twist (TWIST1) (NM_000474) Human Over-expression Lysate

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SKU
LY424701
Transient overexpression lysate of twist homolog 1 (Drosophila) (TWIST1) (as WB positive control)
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC202920
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_000474, NP_000465
Synonyms ACS3; bHLHa38; BPES2; BPES3; CRS; CRS1; CSO; SCS; SWCOS; TWIST
Predicted MW 20.8 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_000465
Locus ID 7291
Cytogenetics 7p21.1
Protein Families WB
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Other "Twist" proteins (3)
SKU Description Size Price
PH302920 TWIST1 MS Standard C13 and N15-labeled recombinant protein (NP_000465) 10 ug
$3,360.00
LC424701 Lyophilized TWIST1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP302920 Recombinant protein of human twist homolog 1 (Drosophila) (TWIST1), 20 µg 20 ug
$737.00

file-alt Citations

TWIST1-WDR5-Hottip regulates Hoxa9 chromatin to facilitate prostate cancer metastasis
Malek, Gajula, Williams et al
Cancer Res. (2017)
AI Summary: OriGene products, specifically shRNA constructs targeting mouse Hoxa9, human HOXA9, mouse Wdr5, and human WDR5, were used for gene knockdown experiments to investigate the roles of these genes in prostate cancer metastasis in this study.
Snippet: Page 7. 7 Retroviral experiments The shRNA constructs against mouse Hoxa9, human HOXA9, mouse Wdr5 and human WDR5 were used according to the manufacturer's instructions (OriGene). Cells were transduced with ... arrays were purchased from OriGene. on May ... All 2 versions Cite SaveSaving
The emerging role of Twist proteins in hematopoietic cells and hematological malignancies
Merindol, Riquet, Szablewski et al
Blood Cancer J (2014) 4, e206 DOI: 10.1038/bcj.2014.22
Cited by 38
AI Summary: OriGene’s Lymphoma cDNA Array II (LYRT102) was used in this study to perform real-time Q-PCR analysis on lymphoma samples, enabling investigation of Twist1–2 expression in B-cell lymphoma.
Snippet: With the aim of investigating a possible role of Twist1" style="font-weight: bold;">Twist1–2 in B-cell lymphoma, we performed real-time Q-PCR on samples derived from Origene (Lymphoma cDNA Array II LYRT102, OriGene Technologies, Inc., Rockville, MD, USA) (Figure 3). A description of each sample ... Cite SaveSaving
Showing 1-2 of 2 papers.
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