Twist (TWIST1) (NM_000474) Human Tagged ORF Clone
$450.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | Twist |
| Synonyms | ACS3; bHLHa38; BPES2; BPES3; CRS; CRS1; CSO; SCS; SWCOS; TWIST |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-RsrII Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_000474 |
| ORF Size | 606 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_000474.4 |
| RefSeq Size | 1669 bp |
| RefSeq ORF | 609 bp |
| Locus ID | 7291 |
| UniProt ID | Q15672 |
| Cytogenetics | 7p21.1 |
| Protein Families | Druggable Genome |
| MW | 21 kDa |
| Summary | This gene encodes a basic helix-loop-helix (bHLH) transcription factor that plays an important role in embryonic development. The encoded protein forms both homodimers and heterodimers that bind to DNA E box sequences and regulate the transcription of genes involved in cranial suture closure during skull development. This protein may also regulate neural tube closure, limb development and brown fat metabolism. This gene is hypermethylated and overexpressed in multiple human cancers, and the encoded protein promotes tumor cell invasion and metastasis, as well as metastatic recurrence. Mutations in this gene cause Saethre-Chotzen syndrome in human patients, which is characterized by craniosynostosis, ptosis and hypertelorism. provided by RefSeq, Jul 2020 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_000474" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC202920L1 | Lenti ORF clone of Human twist homolog 1 (Drosophila) (TWIST1), Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC202920L2 | Lenti ORF clone of Human twist homolog 1 (Drosophila) (TWIST1), mGFP tagged | 10 ug |
$750.00
|
|
| RC202920L3 | Lenti ORF clone of Human twist homolog 1 (Drosophila) (TWIST1), Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC202920L4 | Lenti ORF clone of Human twist homolog 1 (Drosophila) (TWIST1), mGFP tagged | 10 ug |
$750.00
|
|
| RG202920 | TWIST1 (tGFP-tagged) - Human twist homolog 1 (Drosophila) (TWIST1) | 10 ug |
$489.00
$650.00
|
|
| SC321467 | TWIST1 (untagged)-Human twist homolog 1 (Drosophila) (TWIST1) | 10 ug |
$300.00
|
|
Citations
Twist1 Promotes Endothelial Phenotypic Transition and Unstable Plaque Phenotype During Atherosclerosis
Dy, Lehman, Henson et al
Arterioscler Thromb Vasc Biol (2026) 46 (10), e324428-e324428 DOI: 10.1161/atvbaha.126.324428
Dy, Lehman, Henson et al
Arterioscler Thromb Vasc Biol (2026) 46 (10), e324428-e324428 DOI: 10.1161/atvbaha.126.324428
AI Summary: In this study, the researchers used the Human TWIST1 Tagged ORF Clone (SKU RC202920) from OriGene to overexpress TWIST1 in human coronary artery endothelial cells, enabling them to investigate the gene’s role in endothelial phenotypic transition and plaque instability during atherosclerosis.
Snippet: he Zymo DNA Clean &Concentrator-5 Kit (D4003;Zymo Research) was used on the suspended open backbone before continuing with assembly. Human TWIST1 was obtained from Origene (NM_000474 Human Tagged ORF Clone, SKU RC202920;Origene) and clone
The Role of Twist1 in Chronic Pancreatitis-Associated Pancreatic Stellate Cells
Geister, Ard, Patel et al
Am J Pathol (2024)
Geister, Ard, Patel et al
Am J Pathol (2024)
AI Summary: OriGene’s hTwist1 plasmid in the pCMV6-Entry vector (#RC202920) was used to overexpress Twist1 in human pancreatic stellate cells, enabling the study of Twist1’s role in regulating MMP-9 expression and ECM remodeling in chronic pancreatitis.
Snippet: wist1 (hTwist1) in pCMV6-Entry vector was obtained from OriGene (#RC202920;Rockville, MD). The hTwist1 plasmid was inserted into DH10B-competent E. coli via heat shock transformation and then plated onto LB agar plates with kanamycin (50 μg/mL). pCMV6-Entry vector was used as a control. Luciferase Assay Using hPaSCs
Root Extract of Trichosanthes kirilowii Suppresses Metastatic Activity of EGFR TKI-Resistant Human Lung Cancer Cells by Inhibiting Src-Mediated EMT
Park, Park
Nutr Cancer (2023)
Park, Park
Nutr Cancer (2023)
Cited by 7
AI Summary: OriGene’s Myc-DDK-tagged Twist1 vector (#RC202920) was used in this study to transfect lung cancer cells, enabling the researchers to investigate the role of Twist1 in epithelial-mesenchymal transition and metastasis regulated by the Trichosanthes kirilowii root extract.
Snippet: PC9/ER cells (3 × 105 cells) were seeded in 6-well plates and stabilized overnight. These cells were transfected with 1 μg pcSrc527 [a vector containing c-Src Y527F (constitutively active form of c-Src), Addgene, #17675], or Twist1 (Myc-DDK-tagged) vector (#RC202920;OriGene, Rockville, MD, USA) using Lipofectamine 2000. …
Overexpression of the nucleoporin Nup88 stimulates migration and invasion of HeLa cells
Makise, Uchimura, Higashi et al
Histochem Cell Biol (2021)
Makise, Uchimura, Higashi et al
Histochem Cell Biol (2021)
Cited by 11
AI Summary: OriGene’s DDK-tagged Twist1 construct (#RC202920) was used in this study to investigate the molecular mechanisms underlying Nup88-induced migration and invasion in HeLa cells.
Snippet: A construct with DDK-tagged Twist1 (#RC202920) was purchased from OriGene (Rockville, MD, USA). Rabbit polyclonal anti-GFP (#598) and anti-α-Tubulin (#PM054) were purchased from Medical & Biological Laboratories (Tokyo, Japan)
TEAD4 Induced Positive Feedback Activation of E2R/YAP1 Axis and Promoted Epithelial-mesenchymal Transition through Up-regulating TWIST1 Directly in HCC
Liu, Ke, Shang et al
Research Square (2021)
Liu, Ke, Shang et al
Research Square (2021)
PLCε regulates prostate cancer mitochondrial oxidative metabolism and migration via upregulation of Twist1
Fan, Fan, Wang et al
J. Exp. Clin. Cancer Res. (2019) 38 (1), 337 DOI: 10.1186/s13046-019-1323-8
Fan, Fan, Wang et al
J. Exp. Clin. Cancer Res. (2019) 38 (1), 337 DOI: 10.1186/s13046-019-1323-8
AI Summary: In this study, OriGene products—specifically the PLCε shRNA lentiviral particles (TL312431V) and Twist1 antibody (TA806739)—were used to knock down PLCε expression and detect Twist1 protein levels, respectively, enabling the authors to elucidate the regulatory role of PLCε in prostate cancer cell metabolism and migration.
Snippet: Metabolic rewiring is a common feature of many cancer types, including prostate cancer (PCa). Alterations in master genes lead to mitochondrial metabolic rewiring and provide an appealing target to inhibit cancer progression and improve survival. Phospholipase C (PLC)ε is a regulator of tumor generation and progression. However, its role in mitochondrial metabolism remains unclear. ...
Showing 1-6 of 12 papers.
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