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GDF 5 (GDF5) (NM_000557) Human Over-expression Lysate

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SKU
LY424647
Transient overexpression lysate of growth differentiation factor 5 (GDF5) (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC207105
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_000557, NP_000548
Synonyms BDA1C; BMP-14; BMP14; CDMP1; DUPANS; LAP-4; LAP4; OS5; SYM1B; SYNS2
Predicted MW 55.4 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_000548
Locus ID 8200
Cytogenetics 20q11.22
Protein Families WB
Protein Pathways Cytokine-cytokine receptor interaction, TGF-beta signaling pathway
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Documents
Resources
Other "GDF 5" proteins (1)
SKU Description Size Price
LC424647 Lyophilized GDF5 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00

file-alt Citations

Nonviral Gene Delivery of Growth and Differentiation Factor 5 to Human Mesenchymal Stem Cells Injected into a 3D Bovine Intervertebral Disc Organ Culture System
Bucher, Gazdhar, Benneker et al
Stem Cells Int (2013) 2013, 326828 DOI: 10.1155/2013/326828
Cited by 47
AI Summary: In this study, OriGene’s TrueClone GDF5 cDNA (Cat# SC119975) was used to enable nonviral gene delivery of GDF5 into human mesenchymal stem cells, facilitating their differentiation and supporting disc regeneration in a 3D intervertebral disc organ culture model.
FigureFigureFigure
Showing 1-1 of 1 papers.
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