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GDF 5 (GDF5) (NM_000557) Human Tagged ORF Clone

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SKU
RC207105
GDF5 (Myc-DDK-tagged)-Human growth differentiation factor 5 (GDF5)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $466.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "GDF 5 or NM_000557"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol GDF 5
Synonyms BDA1C; BMP-14; BMP14; CDMP1; DUPANS; LAP-4; LAP4; OS5; SYM1B; SYNS2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC207105 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGACTCCCCAAACTCCTCACTTTCTTGCTTTGGTACCTGGCTTGGCTGGACCTGGAATTCATCTGCA
CTGTGTTGGGTGCCCCTGACTTGGGCCAGAGACCCCAGGGGACCAGGCCAGGATTGGCCAAAGCAGAGGC
CAAGGAGAGGCCCCCCCTGGCCCGGAACGTCTTCAGGCCAGGGGGTCACAGCTATGGTGGGGGGGCCACC
AATGCCAATGCCAGGGCAAAGGGAGGCACCGGGCAGACAGGAGGCCTGACACAGCCCAAGAAGGATGAAC
CCAAAAAGCTGCCCCCCAGACCGGGCGGCCCTGAACCCAAGCCAGGACACCCTCCCCAAACAAGGCAGGC
TACAGCCCGGACTGTGACCCCAAAAGGACAGCTTCCCGGAGGCAAGGCACCCCCAAAAGCAGGATCTGTC
CCCAGCTCCTTCCTGCTGAAGAAGGCCAGGGAGCCCGGGCCCCCACGAGAGCCCAAGGAGCCGTTTCGCC
CACCCCCCATCACACCCCACGAGTACATGCTCTCGCTGTACAGGACGCTGTCCGATGCTGACAGAAAGGG
AGGCAACAGCAGCGTGAAGTTGGAGGCTGGCCTGGCCAACACCATCACCAGCTTTATTGACAAAGGGCAA
GATGACCGAGGTCCCGTGGTCAGGAAGCAGAGGTACGTGTTTGACATTAGTGCCCTGGAGAAGGATGGGC
TGCTGGGGGCCGAGCTGCGGATCTTGCGGAAGAAGCCCTCGGACACGGCCAAGCCAGCGGCCCCCGGAGG
CGGGCGGGCTGCCCAGCTGAAGCTGTCCAGCTGCCCCAGCGGCCGGCAGCCGGCCTCCTTGCTGGATGTG
CGCTCCGTGCCAGGCCTGGACGGATCTGGCTGGGAGGTGTTCGACATCTGGAAGCTCTTCCGAAACTTTA
AGAACTCGGCCCAGCTGTGCCTGGAGCTGGAGGCCTGGGAACGGGGCAGGGCCGTGGACCTCCGTGGCCT
GGGCTTCGACCGCGCCGCCCGGCAGGTCCACGAGAAGGCCCTGTTCCTGGTGTTTGGCCGCACCAAGAAA
CGGGACCTGTTCTTTAATGAGATTAAGGCCCGCTCTGGCCAGGACGATAAGACCGTGTATGAGTACCTGT
TCAGCCAGCGGCGAAAACGGCGGGCCCCACTGGCCACTCGCCAGGGCAAGCGACCCAGCAAGAACCTTAA
GGCTCGCTGCAGTCGGAAGGCACTGCATGTCAACTTCAAGGACATGGGCTGGGACGACTGGATCATCGCA
CCCCTTGAGTACGAGGCTTTCCACTGCGAGGGGCTGTGCGAGTTCCCATTGCGCTCCCACCTGGAGCCCA
CGAATCATGCAGTCATCCAGACCCTGATGAACTCCATGGACCCCGAGTCCACACCACCCACCTGCTGTGT
TCCCACGCGGCTGAGTCCCATCAGCATCCTCTTCATTGACTCTGCCAACAACGTGGTGTATAAGCAGTAT
GAGGACATGGTCGTGGAGTCGTGTGGCTGCAGG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC207105 protein sequence
Red=Cloning site Green=Tags(s)

MRLPKLLTFLLWYLAWLDLEFICTVLGAPDLGQRPQGTRPGLAKAEAKERPPLARNVFRPGGHSYGGGAT
NANARAKGGTGQTGGLTQPKKDEPKKLPPRPGGPEPKPGHPPQTRQATARTVTPKGQLPGGKAPPKAGSV
PSSFLLKKAREPGPPREPKEPFRPPPITPHEYMLSLYRTLSDADRKGGNSSVKLEAGLANTITSFIDKGQ
DDRGPVVRKQRYVFDISALEKDGLLGAELRILRKKPSDTAKPAAPGGGRAAQLKLSSCPSGRQPASLLDV
RSVPGLDGSGWEVFDIWKLFRNFKNSAQLCLELEAWERGRAVDLRGLGFDRAARQVHEKALFLVFGRTKK
RDLFFNEIKARSGQDDKTVYEYLFSQRRKRRAPLATRQGKRPSKNLKARCSRKALHVNFKDMGWDDWIIA
PLEYEAFHCEGLCEFPLRSHLEPTNHAVIQTLMNSMDPESTPPTCCVPTRLSPISILFIDSANNVVYKQY
EDMVVESCGCR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_000557
ORF Size 1503 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_000557.5
RefSeq Size 2344 bp
RefSeq ORF 1506 bp
Locus ID 8200
UniProt ID P43026, F1T0J1
Cytogenetics 20q11.22
Protein Families Adult stem cells, Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Secreted Protein, Stem cell relevant signaling - TGFb/BMP signaling pathway
Protein Pathways Cytokine-cytokine receptor interaction, TGF-beta signaling pathway
MW 55.4 kDa
Summary This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates the development of numerous tissue and cell types, including cartilage, joints, brown fat, teeth, and the growth of neuronal axons and dendrites. Mutations in this gene are associated with acromesomelic dysplasia, brachydactyly, chondrodysplasia, multiple synostoses syndrome, proximal symphalangism, and susceptibility to osteoarthritis. provided by RefSeq, Aug 2016
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"NM_000557" in other vectors (6)
SKU Description Size Price
RC207105L1 Lenti ORF clone of Human growth differentiation factor 5 (GDF5), Myc-DDK-tagged 10 ug
$766.00
RC207105L2 Lenti ORF clone of Human growth differentiation factor 5 (GDF5), mGFP tagged 10 ug
$766.00
RC207105L3 Lenti ORF clone of Human growth differentiation factor 5 (GDF5), Myc-DDK-tagged 10 ug
$766.00
RC207105L4 Lenti ORF clone of Human growth differentiation factor 5 (GDF5), mGFP tagged 10 ug
$766.00
RG207105 GDF5 (tGFP-tagged) - Human growth differentiation factor 5 (GDF5) 10 ug
$489.00 $665.00
SC125956 GDF5 (untagged)-Human growth differentiation factor 5 (GDF5) 10 ug
$467.00

Plasmid Map for RC207105

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC207105
Circular map for RC207105

file-alt Citations

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