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IMPDH2 (NM_000884) Human Over-expression Lysate

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SKU
LY424469
Transient overexpression lysate of IMP (inosine monophosphate) dehydrogenase 2 (IMPDH2) (as WB positive control)
  $436.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC202977
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_000884, NP_000875
Synonyms IMPD2; IMPDH-II
Predicted MW 55.8 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_000875
Locus ID 3615
Cytogenetics 3p21.31
Protein Families WB
Protein Pathways Drug metabolism - other enzymes, Metabolic pathways, Purine metabolism
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Other "IMPDH2" proteins (3)
SKU Description Size Price
PH302977 IMPDH2 MS Standard C13 and N15-labeled recombinant protein (NP_000875) 10 ug
$3,360.00
LC424469 Lyophilized IMPDH2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP302977 Recombinant protein of human IMP (inosine monophosphate) dehydrogenase 2 (IMPDH2), 20 µg 20 ug
$867.00

file-alt Citations

Overexpression of inosine 5'-monophosphate dehydrogenase type II mediates chemoresistance to human osteosarcoma cells
Fellenberg, Kunz, Sähr et al
PLoS One (2010) 5 (8), e12179 DOI: 10.1371/journal.pone.0012179
Cited by 43
AI Summary: OriGene products, specifically the IMPDH2 cDNA clone and IMPDH2 shRNA lentiviral particles, were used to generate osteosarcoma cell lines with IMPDH2 overexpression and knockdown, enabling the study of IMPDH2's role in chemoresistance.
FigureFigureFigure
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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