CLEC18B (NM_001011880) Human Over-expression Lysate
SKU
LY423308
Transient overexpression lysate of C-type lectin domain family 18, member B (CLEC18B) (as WB positive control)
$665.00
5 Days*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC215966 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_001011880, NP_001011880 |
| Synonyms | MRCL2 |
| Predicted MW | 50.3 kDa |
| Components | 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer |
| Storage | The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. |
| Shipping | Dry Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). |
| Reference Data | |
| RefSeq | NP_001011880 |
| Locus ID | 497190 |
| Cytogenetics | 16q23.1 |
| Protein Families | WB |
Reviews
Documents
| Product Manuals |
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Resources
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Other "CLEC18B" proteins (2)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| LC423308 | Lyophilized CLEC18B HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| TP761742 | Purified recombinant protein of Human C-type lectin domain family 18, member B (CLEC18B), full length, with N-terminal His tag, expressed in E. coli, 50ug | 50 ug |
$270.00
|
|
Citations
Overexpression of CLEC18B Associates With the Proliferation, Migration, and Prognosis of Glioblastoma
Guo, Zhao, Li et al
ASN Neuro (2018) 10, 1759091418781949 DOI: 10.1177/1759091418781949
Guo, Zhao, Li et al
ASN Neuro (2018) 10, 1759091418781949 DOI: 10.1177/1759091418781949
AI Summary: In this study, the CLEC18B antibody from OriGene Technologies was used for Western blot analysis to assess CLEC18B protein levels in glioblastoma cells, helping to elucidate its role in tumor proliferation and prognosis.
Snippet: Equal amount (20 lg/lane) of protein samples were separated by 10% to 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and then transferred onto polyvinylidene fluoride membranes. The membranes were incubated overnight with the primary antibodies including CLEC18B (1:1000;OriGene Technologies Inc., Rockville, MD, USA), nucleus b-catenin (1:1000), Histone (1:1000), C-Myc (1:1000), and Cyclin D1 (1:1000;Cell Signaling Technology, Danvers, MA, USA)
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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