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VEGFA (NM_001033756) Human Over-expression Lysate

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SKU
LY422419
Transient overexpression lysate of vascular endothelial growth factor A (VEGFA), transcript variant 7 (as WB positive control)
  • LY422419 is the same product as LY425581.
Size:
100 ug 20 ug
Formulation:
Liquid Lyophilized
  $436.00
5 Days*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC217312
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001033756, NP_001028928
Synonyms MVCD1; VEGF; VPF
Predicted MW 40.5 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_001028928
Locus ID 7422
Cytogenetics 6p21.1
Protein Families WB
Protein Pathways Bladder cancer, Cytokine-cytokine receptor interaction, Focal adhesion, mTOR signaling pathway, Pancreatic cancer, Pathways in cancer, Renal cell carcinoma, VEGF signaling pathway
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Other "VEGFA" proteins (9)
SKU Description Size Price
LC422419 Lyophilized VEGFA HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC422435 Lyophilized VEGFA HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC422439 Lyophilized VEGFA HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY422435 Transient overexpression lysate of vascular endothelial growth factor A (VEGFA), transcript variant 1 (as WB positive control) 100 ug
$436.00
LY422439 Transient overexpression lysate of vascular endothelial growth factor A (VEGFA), transcript variant 6 (as WB positive control) 100 ug
$436.00
TP723804 Purified recombinant protein of Human vascular endothelial growth factor A (VEGFA), transcript variant 6 10 ug
$460.00
TP723809 Purified recombinant protein of Human vascular endothelial growth factor A (VEGFA), transcript variant 2 10 ug
$460.00
TP780001 Recombinant protein of human Vascular endothelial growth factor A (VEGF) produced in Pichia Pastoris. 50 ug
$540.00
TP780002 Purified recombinant protein of Human vascular endothelial growth factor A (VEGFA), transcript variant 1, expressed in Pichia Pastoris, 200ug. 200 ug
$1,040.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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