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VEGFA (NM_001033756) Human Over-expression Lysate

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SKU
LC422419
Lyophilized VEGFA HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC217312
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001033756, NP_001028928
Synonyms MVCD1; VEGF; VPF
Predicted MW 40.5 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_001028928
Locus ID 7422
Cytogenetics 6p21.1
Protein Families WB
Protein Pathways Bladder cancer, Cytokine-cytokine receptor interaction, Focal adhesion, mTOR signaling pathway, Pancreatic cancer, Pathways in cancer, Renal cell carcinoma, VEGF signaling pathway
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Other "VEGFA" proteins (9)
SKU Description Size Price
LC422435 Lyophilized VEGFA HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC422439 Lyophilized VEGFA HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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LY422419 Transient overexpression lysate of vascular endothelial growth factor A (VEGFA), transcript variant 7 (as WB positive control) 100 ug
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LY422435 Transient overexpression lysate of vascular endothelial growth factor A (VEGFA), transcript variant 1 (as WB positive control) 100 ug
$436.00
LY422439 Transient overexpression lysate of vascular endothelial growth factor A (VEGFA), transcript variant 6 (as WB positive control) 100 ug
$436.00
TP723804 Purified recombinant protein of Human vascular endothelial growth factor A (VEGFA), transcript variant 6 10 ug
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TP723809 Purified recombinant protein of Human vascular endothelial growth factor A (VEGFA), transcript variant 2 10 ug
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TP780001 Recombinant protein of human Vascular endothelial growth factor A (VEGF) produced in Pichia Pastoris. 50 ug
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TP780002 Purified recombinant protein of Human vascular endothelial growth factor A (VEGFA), transcript variant 1, expressed in Pichia Pastoris, 200ug. 200 ug
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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