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CPEB1 (NM_001079535) Human Over-expression Lysate

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SKU
LY421523
Transient overexpression lysate of cytoplasmic polyadenylation element binding protein 1 (CPEB1), transcript variant 3 (as WB positive control)
  • LY421523 is the same product as LY425886.
Size:
100 ug 20 ug
Formulation:
Liquid Lyophilized
  $436.00
5 Days*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC206406
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001079535, NP_001073003
Synonyms CPE-BP1; CPEB; CPEB-1; h-CPEB; hCPEB-1
Predicted MW 53.6 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_001073003
Locus ID 64506
Cytogenetics 15q25.2
Protein Families WB
Protein Pathways Dorso-ventral axis formation, Oocyte meiosis, Progesterone-mediated oocyte maturation
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Other "CPEB1" proteins (12)
SKU Description Size Price
PH306406 CPEB1 MS Standard C13 and N15-labeled recombinant protein (NP_001073003) 10 ug
$3,360.00
PH313337 CPEB1 MS Standard C13 and N15-labeled recombinant protein (NP_001073002) 10 ug
$3,360.00
LC410792 Lyophilized CPEB1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC421521 Lyophilized CPEB1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC421522 Lyophilized CPEB1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC421523 Lyophilized CPEB1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY410792 Transient overexpression lysate of cytoplasmic polyadenylation element binding protein 1 (CPEB1), transcript variant 1 (as WB positive control) 100 ug
$436.00
LY421521 Transient overexpression lysate of cytoplasmic polyadenylation element binding protein 1 (CPEB1), transcript variant 2 (as WB positive control) 100 ug
$665.00
LY421522 Transient overexpression lysate of cytoplasmic polyadenylation element binding protein 1 (CPEB1), transcript variant 4 (as WB positive control) 100 ug
$665.00
TP306406 Recombinant protein of human cytoplasmic polyadenylation element binding protein 1 (CPEB1), transcript variant 3, 20 µg 20 ug
$867.00
TP313337 Recombinant protein of human cytoplasmic polyadenylation element binding protein 1 (CPEB1), transcript variant 4, 20 µg 20 ug
$867.00
TP761390 Purified recombinant protein of Human cytoplasmic polyadenylation element binding protein 1 (CPEB1), transcript variant 1, full length, with N-terminal GST and C-terminal HIS tag, expressed in E. coli, 50ug 50 ug
$270.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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