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NeuN (RBFOX3) (NM_001082575) Human Over-expression Lysate

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SKU
LY421190
Transient overexpression lysate of hexaribonucleotide binding protein 3 (HRNBP3) (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC224826
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001082575, NP_001076044
Synonyms FOX-3; FOX3; HRNBP3; NEUN
Predicted MW 33.7 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_001076044
Locus ID 146713
Cytogenetics 17q25.3
Protein Families WB
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Documents
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Other "NeuN" proteins (1)
SKU Description Size Price
LC421190 Lyophilized RBFOX3 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00

file-alt Citations

Brain-reactive autoantibodies in neuropsychiatric systemic lupus erythematosus
Cocco, Manca, Corda et al
Front Immunol (2023) 14, 1157149 DOI: 10.3389/fimmu.2023.1157149
Cited by 32
AI Summary: OriGene’s RBFOX3-HEK293T cell lysates (catalogue number LC421190) were used in western blot experiments to assess whether patient sera recognized the NeuN protein, helping to determine that NeuN was not the target autoantigen for brain-reactive autoantibodies in NPSLE.
Snippet: …Western blot Western blot (WB) was performed using either HEK293T and RBFOX3-HEK293T cells transient overexpression lysates (Origene, catalogue number LC421190), which do not or do express the RBFOXP3 gene (encoding the NeuN protein), respectively. Lysates were transferred to polyvinylidene difluoride (PVDF) membranes for 1;- Western blot (WB) was performed using either HEK293T and RBFOX3-HEK293T cells transient overexpression lysates (Origene, catalogue number LC421190), which do
FigureFigureFigure
Showing 1-1 of 1 papers.
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