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NeuN (RBFOX3) (NM_001082575) Human Over-expression Lysate

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SKU
LC421190
Lyophilized RBFOX3 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC224826
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001082575, NP_001076044
Synonyms FOX-3; FOX3; HRNBP3; NEUN
Predicted MW 33.7 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_001076044
Locus ID 146713
Cytogenetics 17q25.3
Protein Families WB
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Other "NeuN" proteins (1)
SKU Description Size Price
LY421190 Transient overexpression lysate of hexaribonucleotide binding protein 3 (HRNBP3) (as WB positive control) 100 ug
$436.00

file-alt Citations

Brain-reactive autoantibodies in neuropsychiatric systemic lupus erythematosus
Cocco, Manca, Corda et al
Front Immunol (2023) 14, 1157149 DOI: 10.3389/fimmu.2023.1157149
Cited by 32
AI Summary: OriGene’s RBFOX3-HEK293T cell lysates (catalogue number LC421190) were used in western blot experiments to assess whether patient sera recognized the NeuN protein, helping to determine that NeuN was not the target autoantigen for brain-reactive autoantibodies in NPSLE.
Snippet: …Western blot Western blot (WB) was performed using either HEK293T and RBFOX3-HEK293T cells transient overexpression lysates (Origene, catalogue number LC421190), which do not or do express the RBFOXP3 gene (encoding the NeuN protein), respectively. Lysates were transferred to polyvinylidene difluoride (PVDF) membranes for 1;- Western blot (WB) was performed using either HEK293T and RBFOX3-HEK293T cells transient overexpression lysates (Origene, catalogue number LC421190), which do
FigureFigureFigure
Showing 1-1 of 1 papers.
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