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TRIM32 (NM_001099679) Human Over-expression Lysate

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SKU
LY420491
Transient overexpression lysate of tripartite motif-containing 32 (TRIM32), transcript variant 2 (as WB positive control)
  • LY420491 is the same product as LY426077.
Size:
100 ug 20 ug
Formulation:
Liquid Lyophilized
  $665.00
5 Days*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC223796
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001099679, NP_001093149
Synonyms BBS11; HT2A; LGMD2H; LGMDR8; TATIP
Predicted MW 72 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_001093149
Locus ID 22954
Cytogenetics 9q33.1
Protein Families WB
Protein Pathways Ubiquitin mediated proteolysis
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Other "TRIM32" proteins (7)
SKU Description Size Price
PH301289 TRIM32 MS Standard C13 and N15-labeled recombinant protein (NP_036342) 10 ug
$3,360.00
PH323796 TRIM32 MS Standard C13 and N15-labeled recombinant protein (NP_001093149) 10 ug
$3,360.00
LC402167 Lyophilized TRIM32 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC420491 Lyophilized TRIM32 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY402167 Transient overexpression lysate of tripartite motif-containing 32 (TRIM32), transcript variant 1 (as WB positive control) 100 ug
$436.00
TP301289 Recombinant protein of human tripartite motif-containing 32 (TRIM32), transcript variant 1, 20 µg 20 ug
$737.00
TP323796 Purified recombinant protein of Homo sapiens tripartite motif-containing 32 (TRIM32), transcript variant 2, 20 µg 20 ug
$737.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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