OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

PACS2 (NM_001100913) Human Over-expression Lysate

Be the first to review this product
SKU
LY420308
Transient overexpression lysate of phosphofurin acidic cluster sorting protein 2 (PACS2), transcript variant 1 (as WB positive control)
  $665.00
5 Days*
Bulk/Customize

Frequently Bought Together (3)

TA339235 Rabbit Polyclonal Anti-PACS2
100 ul
$585.00
TA811000S beta Actin Mouse Monoclonal Antibody, Clone OTI1, Loading Control 30 ul
$200.00
TA592569 DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
100 ul
$519.00

Other products for "PACS2 or NM_001100913"

Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC218762
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001100913, NP_001094383
Synonyms EIEE66; PACS-2; PACS1L
Predicted MW 98 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_001094383
Locus ID 23241
Cytogenetics 14q32.33
Protein Families WB
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
Other "PACS2" proteins (5)
SKU Description Size Price
PH318762 PACS2 MS Standard C13 and N15-labeled recombinant protein (NP_001094383) 10 ug
$3,360.00
LC414719 Lyophilized PACS2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC420308 Lyophilized PACS2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY414719 Transient overexpression lysate of phosphofurin acidic cluster sorting protein 2 (PACS2), transcript variant 2 (as WB positive control) 100 ug
$436.00
TP318762 Recombinant protein of human phosphofurin acidic cluster sorting protein 2 (PACS2), transcript variant 1, 20 µg 20 ug
$867.00

file-alt Citations

PACS2-TRPV1 axis is required for ER-mitochondrial tethering during ER stress and lung fibrosis
Knoell, Chillappagari, Knudsen et al
Cell Mol Life Sci (2022) 79 (3), 151 DOI: 10.1007/s00018-022-04189-2
Cited by 27
AI Summary: OriGene’s PACS2-GFP plasmid was used for transient transfection to study the role of PACS2 in ER-mitochondrial tethering during ER stress and lung fibrosis.
Snippet: ssue culture plates in full medium based on Dulbecco’s modified Eagle medium/Nutrient Mixture F-12 (DMEM-F12 Medium). Transient transfection was performed in a six-well dish using 4 µg PACS2-GFP (OriGene, Rockville, USA) or empty-GFP using 4 µl Lipofectamine 2000 Reagent (Invitrogen, Germany) per well or with non-targeting (NT) or Pacs2 siRNA (both from Santa Cruz) for 48 h as per manufac
FigureFigureFigure
Showing 1-1 of 1 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products