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PACS2 (NM_001100913) Human Tagged ORF Clone

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SKU
RC218762
PACS2 (Myc-DDK-tagged)-Human phosphofurin acidic cluster sorting protein 2 (PACS2), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $818.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "PACS2 or NM_001100913"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PACS2
Synonyms EIEE66; PACS-2; PACS1L
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC218762 representing NM_001100913
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGAGCGAGGCCGCCTCGGCCTCCCCGGCGCGCCCGGCGCGCTCAACACGCCCGTGCCCATGAACC
TGTTCGCCACCTGGGAGGTGGACGGCTCCAGCCCCAGCTGCGTGCCCAGGTTGTGCAGCCTGACTCTGAA
GAAGCTGGTGGTCTTCAAGGAGCTGGAGAAGGAGCTGATCTCCGTGGTGATCGCTGTCAAGATGCAGGGC
TCCAAACGAATCCTGCGGTCCCATGAGATTGTGCTGCCCCCCAGTGGACAAGTGGAGACAGACCTGGCCC
TGACCTTCTCCTTGCAGTATCCTCACTTCTTGAAGAGGGAAGGCAACAAGCTTCAGATCATGCTGCAGCG
CAGAAAGCGCTACAAGAACAGAACCATCCTGGGCTACAAGACGCTGGCCGCGGGCTCCATCAGCATGGCT
GAGGTGATGCAACACCCGTCTGAAGGTGGCCAGGTGCTGAGCCTCTGCAGCAGCATCAAGGAGGCCCCCG
TCAAGGCGGCCGAGATCTGGATCGCCTCCCTGTCCAGCCAGCCCATTGACCACGAAGACAGCACCATGCA
GGCCGGCCCCAAGGCCAAGTCCACGGATAACTACTCCGAGGAGGAGTATGAGAGCTTCTCCTCCGAGCAG
GAGGCCAGTGACGACGCCGTGCAGGGGCAGGACTTGGACGAGGACGACTTTGACGTGGGGAAGCCGAAGA
AGCAGCGGAGATCGATTGTAAGAACGACGTCCATGACCAGGCAACAGAACTTCAAGCAGAAAGTGGTAGC
GCTGCTGCGGAGGTTCAAAGTGTCCGACGAGGTCCTGGACTCGGAGCAGGACCCTGCGGAGCACATCCCC
GAGGCAGAGGAGGACCTGGACCTCCTGTATGACACCCTGGACATGGAGCACCCCAGCGACAGCGGCCCCG
ACATGGAGGATGACGACAGCGTCCTCAGCACCCCCAAGCCGAAGCTGCGGCCATACTTTGAAGGCCTGTC
GCACTCGAGCTCGCAGACGGAGATTGGGAGCATCCACAGCGCCCGCAGCCACAAGGAGCCCCCAAGCCCG
GCTGACGTGCCCGAGAAGACGCGGTCCCTGGGAGGCAGGCAGCCGAGCGACAGTGTCTCTGACACGGTGG
CCCTCGGTGTGCCAGGCCCGAGGGAGCACCCTGGACAGCCTGAGGACAGCCCCGAGGCTGAGGCCTCCAC
CCTGGATGTGTTCACGGAGAGGCTGCCGCCCAGCGGGAGGATCACCAAGACAGAGTCCCTTGTCATCCCC
TCCACCAGGTCCGAGGGGAAGCAGGCTGGCCGACGGGGCCGGAGCACATCCTTGAAGGAGCGGCAGGCAG
CACGGCCCCAGAATGAGCGGGCCAACAGCCTGGACAACGAGCGCTGCCCGGACGCCCGGAGCCAGCTACA
GGTGCAGCTGCAGATCCCCAGGAAGACTGTGTATGACCAGCTCAACCACATCCTCATCTCCGATGACCAG
CTTCCCGAAAACATCATCCTTGTCAACACCTCGGACTGGCAGGGGCAGTTCCTCTCCGACGTCCTGCAGA
GGCACACGCTCCCCGTGGTGTGCACGTGCTCTCCTGCGGACGTCCAGGCGGCCTTCAGCACCATCGTCTC
ACGGATACAGAGATACTGCAACTGCAATTCCCAGCCCCCGACCCCCGTGAAGATCGCCGTGGCGGGAGCG
CAGCATTACCTCAGTGCCATCCTGCGGCTCTTTGTGGAGCAGCTGTCCCACAAGACACCCGACTGGCTCG
GCTACATGCGCTTCCTGGTCATCCCACTGGGCTCCCACCCCGTGGCCAGGTACCTAGGCTCCGTGGACTA
CCGCTACAACAACTTCTTCCAGGACCTGGCCTGGAGAGACCTGTTCAACAAGCTGGAGGCCCAGAGTGCG
GTACAGGACACGCCAGACATTGTGTCACGCATCACGCAGTACATCGCAGGGGCCAACTGTGCCCACCAGC
TCCCCATCGCAGAGGCCATGCTGACCTACAAGCAGAAGAGCCCTGACGAAGAGTCCTCCCAAAAGTTCAT
TCCCTTTGTCGGGGTTGTGAAGGTTGGAATTGTGGAGCCATCCTCGGCCACATCAGGCGACTCGGACGAC
GCGGCCCCCTCGGGCTCTGGCACGCTCTCCTCCACCCCGCCGTCCGCATCTCCTGCGGCCAAGGAGGCCT
CACCCACCCCGCCCTCCTCCCCGTCGGTGAGCGGAGGCCTGTCCTCCCCCAGCCAGGGTGTCGGCGCCGA
GCTGATGGGGCTGCAGGTGGACTACTGGACGGCAGCACAGCCTGCGGACAGGAAGAGGGACGCCGAGAAG
AAGGACCTGCCTGTCACCAAAAACACGCTCAAGTGCACTTTCCGGTCCCTCCAGGTCAGCAGGCTGCCCA
GCAGCGGCGAGGCTGCAGCCACGCCCACCATGTCCATGACCGTGGTCACCAAGGAGAAGAACAAGAAGGT
GATGTTTCTGCCCAAGAAAGCGAAGGACAAGGACGTGGAGTCTAAGAGCCAGTGCATTGAGGGCATCAGC
CGGCTCATCTGCACTGCCAGGCAGCAGCAGAACATGCTGCGGGTCCTCATCGACGGCGTGGAGTGCAGCG
ACGTCAAGTTCTTCCAGCTGGCCGCGCAGTGGTCCTCGCACGTGAAGCACTTCCCCATCTGCATCTTCGG
ACACTCCAAGGCCACCTTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC218762 representing NM_001100913
Red=Cloning site Green=Tags(s)

MAERGRLGLPGAPGALNTPVPMNLFATWEVDGSSPSCVPRLCSLTLKKLVVFKELEKELISVVIAVKMQG
SKRILRSHEIVLPPSGQVETDLALTFSLQYPHFLKREGNKLQIMLQRRKRYKNRTILGYKTLAAGSISMA
EVMQHPSEGGQVLSLCSSIKEAPVKAAEIWIASLSSQPIDHEDSTMQAGPKAKSTDNYSEEEYESFSSEQ
EASDDAVQGQDLDEDDFDVGKPKKQRRSIVRTTSMTRQQNFKQKVVALLRRFKVSDEVLDSEQDPAEHIP
EAEEDLDLLYDTLDMEHPSDSGPDMEDDDSVLSTPKPKLRPYFEGLSHSSSQTEIGSIHSARSHKEPPSP
ADVPEKTRSLGGRQPSDSVSDTVALGVPGPREHPGQPEDSPEAEASTLDVFTERLPPSGRITKTESLVIP
STRSEGKQAGRRGRSTSLKERQAARPQNERANSLDNERCPDARSQLQVQLQIPRKTVYDQLNHILISDDQ
LPENIILVNTSDWQGQFLSDVLQRHTLPVVCTCSPADVQAAFSTIVSRIQRYCNCNSQPPTPVKIAVAGA
QHYLSAILRLFVEQLSHKTPDWLGYMRFLVIPLGSHPVARYLGSVDYRYNNFFQDLAWRDLFNKLEAQSA
VQDTPDIVSRITQYIAGANCAHQLPIAEAMLTYKQKSPDEESSQKFIPFVGVVKVGIVEPSSATSGDSDD
AAPSGSGTLSSTPPSASPAAKEASPTPPSSPSVSGGLSSPSQGVGAELMGLQVDYWTAAQPADRKRDAEK
KDLPVTKNTLKCTFRSLQVSRLPSSGEAAATPTMSMTVVTKEKNKKVMFLPKKAKDKDVESKSQCIEGIS
RLICTARQQQNMLRVLIDGVECSDVKFFQLAAQWSSHVKHFPICIFGHSKATF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001100913
ORF Size 2679 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001100913.1, NP_001094383.1
RefSeq Size 6332 bp
RefSeq ORF 2715 bp
Locus ID 23241
UniProt ID Q86VP3
Cytogenetics 14q32.33
MW 98 kDa
Summary Multifunctional sorting protein that controls the endoplasmic reticulum (ER)-mitochondria communication, including the apposition of mitochondria with the ER and ER homeostasis. In addition, in response to apoptotic inducer, translocates BIB to mitochondria, which initiates a sequence of events including the formation of mitochondrial truncated BID, the release of cytochrome c, the activation of caspase-3 thereby causing cell death. May also be involved in ion channel trafficking, directing acidic cluster-containing ion channels to distinct subcellular compartments.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001100913" in other vectors (4)
SKU Description Size Price
RC218762L3 Lenti ORF clone of Human phosphofurin acidic cluster sorting protein 2 (PACS2), transcript variant 1, Myc-DDK-tagged 10 ug
$1,118.00
RC218762L4 Lenti ORF clone of Human phosphofurin acidic cluster sorting protein 2 (PACS2), transcript variant 1, mGFP tagged 10 ug
$1,118.00
RG218762 PACS2 (tGFP-tagged) - Human phosphofurin acidic cluster sorting protein 2 (PACS2), transcript variant 1 10 ug
$1,018.00
SC316844 PACS2 (untagged)-Human phosphofurin acidic cluster sorting protein 2 (PACS2), transcript variant 1 10 ug
$911.00

Plasmid Map for RC218762

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC218762
Circular map for RC218762

file-alt Citations

Feature Article: mTOR complex 2-Akt signaling at mitochondria-associated endoplasmic reticulum membranes (MAM) regulates mitochondrial physiology
Betz, Stracka, Prescianotto-Baschong et al
PNAS (2013) 110 (31), 12526-34 DOI: 10.1073/pnas.1302455110
Cited by 536
AI Summary: OriGene's PACS2-Flag plasmid was used in this study to investigate the role of PACS2 in mTORC2 signaling at mitochondria-associated ER membranes, aiding in the analysis of MAM integrity and mitochondrial function.
Snippet: PACS2-Flag was purchased from Origene. Protein Lysates, Fractionation, and Immunoprecipitation. ... Microscopy. For organelle fluorescence labeling, cells were infected 24 h before harvest with baculovirus expressing ER-GFP or Mito-RFP (BacMAM;Life Technologies).
Showing 1-1 of 1 papers.
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