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RGS8 (NM_001102450) Human Over-expression Lysate

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SKU
LY420151
Transient overexpression lysate of regulator of G-protein signaling 8 (RGS8), transcript variant 2 (as WB positive control)
  • LY420151 is the same product as LY426179.
Size:
100 ug 20 ug
Formulation:
Liquid Lyophilized
  $436.00
5 Days*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC212467
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001102450, NP_001095920
Predicted MW 20.7 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_001095920
Locus ID 85397
Cytogenetics 1q25.3
Protein Families WB
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Other "RGS8" proteins (5)
SKU Description Size Price
PH310236 RGS8 MS Standard C13 and N15-labeled recombinant protein (NP_203131) 10 ug
$3,360.00
LC409583 Lyophilized RGS8 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC420151 Lyophilized RGS8 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY409583 Transient overexpression lysate of regulator of G-protein signaling 8 (RGS8), transcript variant 1 (as WB positive control) 100 ug
$436.00
TP310236 Recombinant protein of human regulator of G-protein signaling 8 (RGS8), transcript variant 1, 20 µg 20 ug
$867.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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