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RGS8 (NM_001102450) Human Tagged ORF Clone

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SKU
RC212467
RGS8 (Myc-DDK-tagged)-Human regulator of G-protein signaling 8 (RGS8), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "RGS8 or NM_001102450"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol RGS8
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC212467 representing NM_001102450
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCCTTACTGATGCCACGCAGGAACAAAGGGATGAGGACTCGACTGGGATGCCTGTCTCACAAGT
CAGACTCGTGTAGTGATTTCACAGCTATTCTTCCAGACAAACCCAACCGCGCTCTCAAGAGATTATCGAC
AGAAGAAGCTACGAGGTGGGCAGATTCCTTTGATGTGCTTCTCTCTCATAAGTATGGGGTGGCTGCATTC
CGTGCCTTCTTGAAGACGGAGTTCAGTGAGGAGAACCTGGAATTCTGGTTGGCCTGTGAGGAGTTCAAGA
AGACCAGGTCAACTGCAAAACTGGTCTCTAAGGCCCATAGGATCTTTGAGGAGTTTGTGGATGTGCAGGC
TCCACGGGAGGTAAACATTGACTTCCAGACCCGAGAAGCCACGAGGAAGAACCTGCAGGAGCCATCCCTG
ACTTGCTTTGACCAAGCCCAAGGAAAAGTACACAGCCTCATGGAGAAAGACTCTTACCCCAGGTTCCTGA
GGTCCAAAATGTACTTAGATCTGCTGTCCCAAAGCCAGAGGAGGCTCAGT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC212467 representing NM_001102450
Red=Cloning site Green=Tags(s)

MAALLMPRRNKGMRTRLGCLSHKSDSCSDFTAILPDKPNRALKRLSTEEATRWADSFDVLLSHKYGVAAF
RAFLKTEFSEENLEFWLACEEFKKTRSTAKLVSKAHRIFEEFVDVQAPREVNIDFQTREATRKNLQEPSL
TCFDQAQGKVHSLMEKDSYPRFLRSKMYLDLLSQSQRRLS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001102450
ORF Size 540 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001102450.3
RefSeq Size 912 bp
RefSeq ORF 543 bp
Locus ID 85397
UniProt ID P57771
Cytogenetics 1q25.3
Protein Families Druggable Genome
MW 20.7 kDa
Summary This gene is a member of the regulator of G protein signaling (RGS) family and encodes a protein with a single RGS domain. Regulator of G protein signaling (RGS) proteins are regulatory and structural components of G protein-coupled receptor complexes. They accelerate transit through the cycle of GTP binding and hydrolysis to GDP, thereby terminating signal transduction, but paradoxically, also accelerate receptor-stimulated activation. provided by RefSeq, Jul 2008
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"NM_001102450" in other vectors (4)
SKU Description Size Price
RC212467L3 Lenti ORF clone of Human regulator of G-protein signaling 8 (RGS8), transcript variant 2, Myc-DDK-tagged 10 ug
$600.00
RC212467L4 Lenti ORF clone of Human regulator of G-protein signaling 8 (RGS8), transcript variant 2, mGFP tagged 10 ug
$600.00
RG212467 RGS8 (tGFP-tagged) - Human regulator of G-protein signaling 8 (RGS8), transcript variant 2 10 ug
$500.00
SC316903 RGS8 (untagged)-Human regulator of G-protein signaling 8 (RGS8), transcript variant 2 10 ug
$330.00

Plasmid Map for RC212467

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC212467
Circular map for RC212467

file-alt Citations

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