OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

BMPR2 (NM_001204) Human Over-expression Lysate

Be the first to review this product
SKU
LY420077
Transient overexpression lysate of bone morphogenetic protein receptor, type II (serine/threonine kinase) (BMPR2) (as WB positive control)
  $436.00
2 Weeks*
Bulk/Customize

Frequently Bought Together (3)

TA323211 Anti-BMPR2 Rabbit Polyclonal Antibody
100 ul
$305.00
TA811000S beta Actin Mouse Monoclonal Antibody, Clone OTI1, Loading Control 30 ul
$200.00
TA592569 DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
100 ul
$519.00

Other products for "BMPR2 or NM_001204"

Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC208673
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001204, NP_001195
Synonyms BMPR-II; BMPR3; BMR2; BRK-3; POVD1; PPH1; T-ALK
Predicted MW 115.2 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_001195
Locus ID 659
Cytogenetics 2q33.1-q33.2
Protein Families WB
Protein Pathways Cytokine-cytokine receptor interaction, TGF-beta signaling pathway
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
Other "BMPR2" proteins (4)
SKU Description Size Price
PH308673 BMPR2 MS Standard C13 and N15-labeled recombinant protein (NP_001195) 10 ug
$3,360.00
LC420077 Lyophilized BMPR2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP308673 Recombinant protein of human bone morphogenetic protein receptor, type II (serine/threonine kinase) (BMPR2), 20 µg 20 ug
$737.00
TP720272 Recombinant protein of human bone morphogenetic protein receptor, type II (serine/threonine kinase) (BMPR2) 10 ug
$190.00

file-alt Citations

Hyperactive TGF-β Signaling in Smooth Muscle Cells Exposed to HIV-protein(s) and Cocaine: Role in Pulmonary Vasculopathy
Dalvi, Sharma, Konstantinova et al
Sci Rep (2017) 7 (1), 10433 DOI: 10.1038/s41598-017-10438-3
AI Summary: OriGene's PCMV6-XL5-BMPR2 plasmid and PCMV6-XL5 vector were used to manipulate BMPR2 expression in human pulmonary arterial smooth muscle cells, enabling the study of how BMPR2 downregulation by HIV-proteins and cocaine contributes to hyperactive TGF-β signaling and pulmonary vasculopathy.
Snippet: After 2 days of transfecting HPASMC with either PCMV6-XL5-BMPR2 plasmid or empty PCMV6-XL5 vector (Origene Technologies, Inc., Rockville, MD) as described previously 15 , cells were serum starved for 48 h followed by cocaine and Tat treatment.
FigureFigureFigure
BMP-2/4 and BMP-6/7 differentially utilize cell surface receptors to induce osteoblastic differentiation of human bone marrow-derived mesenchymal stem cells
Lavery, Swain, Falb et al
J Biol Chem (2008) 283 (30), 20948-58 DOI: 10.1074/jbc.M800850200
AI Summary: OriGene products contributed to this study by providing specific siRNAs (OriGene, SR321082, SR304684, SR304685, SR303554, SR321083, SR321084) used for RNA interference-mediated gene knockdown of BMP receptors in human mesenchymal stem cells, enabling the authors to elucidate receptor-specific signaling mechanisms during osteoblastic differentiation.
Showing 1-2 of 2 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products