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BMPR2 (NM_001204) Human Over-expression Lysate

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SKU
LC420077
Lyophilized BMPR2 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC208673
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001204, NP_001195
Synonyms BMPR-II; BMPR3; BMR2; BRK-3; POVD1; PPH1; T-ALK
Predicted MW 115.2 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_001195
Locus ID 659
Cytogenetics 2q33.1-q33.2
Protein Families WB
Protein Pathways Cytokine-cytokine receptor interaction, TGF-beta signaling pathway
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Other "BMPR2" proteins (4)
SKU Description Size Price
PH308673 BMPR2 MS Standard C13 and N15-labeled recombinant protein (NP_001195) 10 ug
$3,360.00
LY420077 Transient overexpression lysate of bone morphogenetic protein receptor, type II (serine/threonine kinase) (BMPR2) (as WB positive control) 100 ug
$436.00
TP308673 Recombinant protein of human bone morphogenetic protein receptor, type II (serine/threonine kinase) (BMPR2), 20 µg 20 ug
$737.00
TP720272 Recombinant protein of human bone morphogenetic protein receptor, type II (serine/threonine kinase) (BMPR2) 10 ug
$190.00

file-alt Citations

Hyperactive TGF-β Signaling in Smooth Muscle Cells Exposed to HIV-protein(s) and Cocaine: Role in Pulmonary Vasculopathy
Dalvi, Sharma, Konstantinova et al
Sci Rep (2017) 7 (1), 10433 DOI: 10.1038/s41598-017-10438-3
AI Summary: OriGene's PCMV6-XL5-BMPR2 plasmid and PCMV6-XL5 vector were used to manipulate BMPR2 expression in human pulmonary arterial smooth muscle cells, enabling the study of how BMPR2 downregulation by HIV-proteins and cocaine contributes to hyperactive TGF-β signaling and pulmonary vasculopathy.
Snippet: After 2 days of transfecting HPASMC with either PCMV6-XL5-BMPR2 plasmid or empty PCMV6-XL5 vector (Origene Technologies, Inc., Rockville, MD) as described previously 15 , cells were serum starved for 48 h followed by cocaine and Tat treatment.
FigureFigureFigure
BMP-2/4 and BMP-6/7 differentially utilize cell surface receptors to induce osteoblastic differentiation of human bone marrow-derived mesenchymal stem cells
Lavery, Swain, Falb et al
J Biol Chem (2008) 283 (30), 20948-58 DOI: 10.1074/jbc.M800850200
AI Summary: OriGene products contributed to this study by providing specific siRNAs (OriGene, SR321082, SR304684, SR304685, SR303554, SR321083, SR321084) used for RNA interference-mediated gene knockdown of BMP receptors in human mesenchymal stem cells, enabling the authors to elucidate receptor-specific signaling mechanisms during osteoblastic differentiation.
Showing 1-2 of 2 papers.
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