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CRMP2 (DPYSL2) (NM_001386) Human Over-expression Lysate

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SKU
LY419992
Transient overexpression lysate of dihydropyrimidinase-like 2 (DPYSL2) (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC209080
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001386, NP_001377
Synonyms CRMP-2; CRMP2; DHPRP2; DRP-2; DRP2; N2A3; ULIP-2; ULIP2
Predicted MW 62.3 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_001377
Locus ID 1808
Cytogenetics 8p21.2
Protein Families WB
Protein Pathways Axon guidance
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Other "CRMP2" proteins (5)
SKU Description Size Price
PH309080 DPYSL2 MS Standard C13 and N15-labeled recombinant protein (NP_001377) 10 ug
$3,360.00
LC419992 Lyophilized DPYSL2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC434367 Lyophilized DPYSL2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY434367 Transient overexpression lysate of dihydropyrimidinase-like 2 (DPYSL2), transcript variant 1 (as WB positive control) 100 ug
$665.00
TP309080 Purified recombinant protein of Homo sapiens dihydropyrimidinase-like 2 (DPYSL2), 20 µg 20 ug
$867.00

file-alt Citations

Autism-specific maternal autoantibodies recognize critical proteins in developing brain
Braunschweig, Krakowiak, Duncanson et al
Transl Psychiatry (2013) 3, e277 DOI: 10.1038/tp.2013.50
Cited by 263
AI Summary: OriGene products, specifically full-length CRMP1, CRMP2, and YBX1 proteins, were used as key antigens in western blot assays to identify autism-specific maternal autoantibodies in this study.
Snippet: μm pore nitrocellulose membrane (GE Healthcare) and a western blot performed using maternal plasma samples reactive to the band region of ... length collapsin response mediator proteins 1 and 2 (CRMP1, CRMP2) and Y-Box-binding protein 1 (YBX1;OriGene, Rockville, MD
FigureFigureFigure
Showing 1-1 of 1 papers.
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