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beta Catenin (CTNNB1) (NM_001904) Human Over-expression Lysate

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SKU
LY419662
Transient overexpression lysate of catenin (cadherin-associated protein), beta 1, 88kDa (CTNNB1), transcript variant 1 (as WB positive control)
  $436.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC208947
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001904, NP_001895
Synonyms armadillo; CTNNB; EVR7; MRD19; NEDSDV
Predicted MW 85.3 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_001895
Locus ID 1499
Cytogenetics 3p22.1
Protein Families WB
Protein Pathways Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Basal cell carcinoma, Colorectal cancer, Endometrial cancer, Focal adhesion, Leukocyte transendothelial migration, Melanogenesis, Pathogenic Escherichia coli infection, Pathways in cancer, Prostate cancer, Thyroid cancer, Tight junction, Wnt signaling pathway
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Other "beta Catenin" proteins (15)
SKU Description Size Price
PH308947 CTNNB1 MS Standard C13 and N15-labeled recombinant protein (NP_001895) 10 ug
$3,360.00
PH312056 CTNNB1 MS Standard C13 and N15-labeled recombinant protein (NP_001091679) 10 ug
$3,360.00
PH314557 CTNNB1 MS Standard C13 and N15-labeled recombinant protein (NP_001091680) 10 ug
$3,360.00
LC419662 Lyophilized CTNNB1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC420557 Lyophilized CTNNB1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC420558 Lyophilized CTNNB1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC426005 Lyophilized CTNNB1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY420557 Transient overexpression lysate of catenin (cadherin-associated protein), beta 1, 88kDa (CTNNB1), transcript variant 2 (as WB positive control) 100 ug
$665.00
LY420558 Transient overexpression lysate of catenin (cadherin-associated protein), beta 1, 88kDa (CTNNB1), transcript variant 3 (as WB positive control) 100 ug
$665.00
LY426005 Transient overexpression lysate of catenin (cadherin-associated protein), beta 1, 88kDa (CTNNB1), transcript variant 2 (as WB positive control) 100 ug
$665.00
TP308947 Recombinant protein of human catenin (cadherin-associated protein), beta 1, 88kDa (CTNNB1), 20 µg 20 ug
$867.00
TP312056 Recombinant protein of human catenin (cadherin-associated protein), beta 1, 88kDa (CTNNB1), 20 µg 20 ug
$867.00
TP314557 Recombinant protein of human catenin (cadherin-associated protein), beta 1, 88kDa (CTNNB1), 20 µg 20 ug
$867.00
TP710027 Recombinant protein of human human catenin (cadherin-associated protein), beta 1(CTNNB1), full length, with C-terminal DDK tag, expressed in sf9 cells 20 ug
$540.00
TP710124 Recombinant protein of human human catenin (cadherin-associated protein), beta 1(CTNNB1), full length, with N-terminal His tag, expressed in sf9 cells 20 ug
$540.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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