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GADD45A (NM_001924) Human Over-expression Lysate

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SKU
LY419659
Transient overexpression lysate of growth arrest and DNA-damage-inducible, alpha (GADD45A) (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC204005
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001924, NP_001915
Synonyms DDIT1; GADD45
Predicted MW 18.3 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_001915
Locus ID 1647
Cytogenetics 1p31.3
Protein Families WB
Protein Pathways Cell cycle, MAPK signaling pathway, p53 signaling pathway
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Other "GADD45A" proteins (4)
SKU Description Size Price
PH304005 GADD45A MS Standard C13 and N15-labeled recombinant protein (NP_001915) 10 ug
$3,360.00
LC419659 Lyophilized GADD45A HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP304005 Recombinant protein of human growth arrest and DNA-damage-inducible, alpha (GADD45A), 20 µg 20 ug
$867.00
TP720561 Recombinant protein of human growth arrest and DNA-damage-inducible, alpha (GADD45A) 10 ug
$340.00

file-alt Citations

An alternative microRNA-mediated post-transcriptional regulation of GADD45A by p53 in human non-small-cell lung cancer cells
Li, Dong, Li et al
Sci Rep (2017) 7 (1), 7153 DOI: 10.1038/s41598-017-07332-3
AI Summary: OriGene products pCMV/AGO2 and pCMV/GADD45A were used in this study to overexpress AGO2 and GADD45A, respectively, enabling the authors to investigate the post-transcriptional regulation of GADD45A by miR-138 in non-small-cell lung cancer cells.
Snippet: used as a negative control (Promega, Madison, WI, USA), pRC/p53 containing the entire p53 coding region was obtained from Sino Biological (Sino Biological Inc, Beijing, China), and pCMV/AGO2 and pCMV/GADD45A were purchased from OriGene (OriGene Technologies ... All 4 versions Cite SaveSaving
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Showing 1-1 of 1 papers.
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