GADD45A (NM_001924) Human Tagged ORF Clone
SKU
RC204005
GADD45A (Myc-DDK-tagged)-Human growth arrest and DNA-damage-inducible, alpha (GADD45A), transcript variant 1
$225.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | GADD45A |
| Synonyms | DDIT1; GADD45 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_001924 |
| ORF Size | 495 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001924.4 |
| RefSeq Size | 1398 bp |
| RefSeq ORF | 498 bp |
| Locus ID | 1647 |
| UniProt ID | P24522 |
| Cytogenetics | 1p31.3 |
| Domains | Ribosomal_L7Ae |
| Protein Families | Druggable Genome, Stem cell - Pluripotency |
| Protein Pathways | Cell cycle, MAPK signaling pathway, p53 signaling pathway |
| MW | 18.3 kDa |
| Summary | This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The DNA damage-induced transcription of this gene is mediated by both p53-dependent and -independent mechanisms. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.provided by RefSeq, Dec 2010 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_001924" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC204005L1 | Lenti ORF clone of Human growth arrest and DNA-damage-inducible, alpha (GADD45A), transcript variant 1, Myc-DDK-tagged | 10 ug |
$525.00
|
|
| RC204005L2 | Lenti ORF clone of Human growth arrest and DNA-damage-inducible, alpha (GADD45A), transcript variant 1, mGFP tagged | 10 ug |
$525.00
|
|
| RC204005L3 | Lenti ORF clone of Human growth arrest and DNA-damage-inducible, alpha (GADD45A), transcript variant 1, Myc-DDK-tagged | 10 ug |
$525.00
|
|
| RC204005L4 | Lenti ORF clone of Human growth arrest and DNA-damage-inducible, alpha (GADD45A), transcript variant 1, mGFP tagged | 10 ug |
$525.00
|
|
| RG204005 | GADD45A (tGFP-tagged) - Human growth arrest and DNA-damage-inducible, alpha (GADD45A), transcript variant 1 | 10 ug |
$425.00
|
|
| SC118947 | GADD45A (untagged)-Human growth arrest and DNA-damage-inducible, alpha (GADD45A), transcript variant 1 | 10 ug |
$150.00
|
|
Citations
EGR1/GADD45α Activation by ROS of Non-Thermal Plasma Mediates Cell Death in Thyroid Carcinoma
Jung, Oh, Chang et al
Cancers (Basel) (2021) 13 (2) DOI: 10.3390/cancers13020351
Jung, Oh, Chang et al
Cancers (Basel) (2021) 13 (2) DOI: 10.3390/cancers13020351
Cited by 32
AI Summary: In this study, OriGene products—specifically the EGR1 overexpression plasmid (RC207307) and EGR1 siRNA (SR304236)—were used to modulate EGR1 expression, enabling the researchers to demonstrate that EGR1 activation mediates non-thermal plasma-induced cell death in thyroid carcinoma cells.
Snippet: ggest that the greater effect of NTPAM in cancer cells may be related to decreased mitochondrial function in cancer cells compared to normal cells at baseline. 2.3. EGR1 Is a Regulator of Thyroid Tumorigenesis To determine the major mechanism of NTPAM in human THCA, we conducted transcriptome analysis of THCA cells with or without 2 h dose-NTPAM treatment for 24 h. As shown in Figure 3A,B, change
GADD45α-targeted suicide gene therapy driven by synthetic CArG promoter E9NS sensitizes NSCLC cells to cisplatin, resveratrol, and radiation regardless of p53 status
Shi, Sutariya, Varghese Gupta et al
Onco Targets Ther (2019) 12, 3161-3170 DOI: 10.2147/OTT.S192061
Shi, Sutariya, Varghese Gupta et al
Onco Targets Ther (2019) 12, 3161-3170 DOI: 10.2147/OTT.S192061
AI Summary: OriGene’s human GADD45α cDNA was used as the template to amplify the GADD45α open reading frame, which was essential for constructing the suicide gene therapy vector evaluated in this study.
Snippet: The pTargetTM Vector (Promega, Madison, WI, USA) was used as the basis for pE9NS.G45α. The human GADD45α opening reading frame (ORF) was amplified from GADD45α cDNA (Origene, MD, USA) using PCR
An alternative microRNA-mediated post-transcriptional regulation of GADD45A by p53 in human non-small-cell lung cancer cells
Li, Dong, Li et al
Sci Rep (2017) 7 (1), 7153 DOI: 10.1038/s41598-017-07332-3
Li, Dong, Li et al
Sci Rep (2017) 7 (1), 7153 DOI: 10.1038/s41598-017-07332-3
AI Summary: OriGene products pCMV/AGO2 and pCMV/GADD45A were used in this study to overexpress AGO2 and GADD45A, respectively, enabling the authors to investigate the post-transcriptional regulation of GADD45A by miR-138 in non-small-cell lung cancer cells.
Snippet: used as a negative control (Promega, Madison, WI, USA), pRC/p53 containing the entire p53 coding region was obtained from Sino Biological (Sino Biological Inc, Beijing, China), and pCMV/AGO2 and pCMV/GADD45A were purchased from OriGene (OriGene Technologies ... All 4 versions Cite SaveSaving
Gadd45a sensitizes medulloblastoma cells to irradiation and suppresses MMP-9-mediated EMT
Asuthkar, Nalla, Gondi et al
Neuro Oncol (2011) 13 (10), 1059-73 DOI: 10.1093/neuonc/nor109
Asuthkar, Nalla, Gondi et al
Neuro Oncol (2011) 13 (10), 1059-73 DOI: 10.1093/neuonc/nor109
Cited by 57
AI Summary: In this study, OriGene’s Gadd45a siRNA and Gadd45a overexpression (Ov-exp) plasmids were used to modulate Gadd45a levels in medulloblastoma cell lines, enabling the authors to demonstrate Gadd45a’s role in sensitizing cells to irradiation and suppressing MMP-9-mediated epithelial-mesenchymal transition.
Showing 1-4 of 4 papers.
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