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GADD45A (NM_001924) Human Untagged Clone

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SKU
SC118947
GADD45A (untagged)-Human growth arrest and DNA-damage-inducible, alpha (GADD45A), transcript variant 1
  $150.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol GADD45A
Synonyms DDIT1; GADD45
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_001924 edited
GAATTCGGCACGAGGGGCTGGGAGGCAGCGGCCCAATTAGTGTCGTGCGGCCCGTGGCGA
GGCGAGGTCCGGGGAGCGAGCGAGCAAGCAAGGCGGGAGGGGTGGCCGGAGCTGCGGCGG
CTGGCACAGGAGGAGGAGCCCGGGCGGGCGAGGGGCGGCCGGAGAGCGCCAGGGCCTGAG
CTGCCGGAGCGGCGCCTGTGAGTGAGTGCAGAAAGCAGGCGCCCGCGCGCTAGCCGTGGC
AGGAGCAGCCCGCACGCCGCGCTCTCTCCCTGGGCGACCTGCAGTTTGCAATATGACTTT
GGAGGAATTCTCGGCTGGAGAGCAGAAGACCGAAAGGATGGATAAGGTGGGGGATGCCCT
GGAGGAAGTGCTCAGCAAAGCCCTGAGTCAGCGCACGATCACTGTCGGGGTGTACGAAGC
GGCCAAGCTGCTCAACGTCGACCCCGATAACGTGGTGTTGTGCCTGCTGGCGGCGGACGA
GGACGACGACAGAGATGTGGCTCTGCAGATCCACTTCACCCTGATCCAGGCGTTTTGCTG
CGAGAACGACATCAACATCCTGCGCGTCAGCAACCCGGGCCGGCTGGCGGAGCTCCTGCT
CTTGGAGACCGACGCTGGCCCCGCGGCGAGCGAGGGCGCCGAGCAGCCCCCGGACCTGCA
CTGCGTGCTGGTGACGAATCCACATTCATCTCAATGGAAGGATCCTGCCTTAAGTCAACT
TATTTGTTTTTGCCGGGAAAGTCGCTACATGGATCAATGGGTTCCAGTGATTAATCTCCC
TGAACGGTGATGGCATCTGAATGAAAATAACTGAACCAAATTGCACTGAAGTTTTTGAAA
TACCTTTGTAGTTACTCAAGCAGTTACTCCCTACACTGATGCAAGGATTACAGAAACTGA
TGCCAAGGGGCTGAGTGAGTTCAACTACATGTTCTGGGGGCCCGGAGATAGATGACTTTG
CAGATGGAAAGAGGTGAAAATGAAGAAGGAAGCTGTGTTGAAACAGAAAAATAAGTCAAA
AGGAACAAAAATTACAAAGAACCATGCAGGAAGGAAAACTATGTATTAATTTAGAATGGT
TGAGTTACATTAAAATAAACCAAATATGTTAAAGTTTAAGTGTGCAGCCATAGTTTGGGT
ATTTTTGGTTTATATGCCCTCAAGTAAAAGAAAAGCCGAAAGGGTTAATCATATTTGAAA
ACCATATTTTATTGTATTTTGATGAGATATTAAATTCTCAAAGTTTTATTATAAATTCTA
CTAAGTTATTTTATGACATGAAAAGTTATTTATGCTATAAATTTTTTGAAACACAATACC
TACAATAAACTGGTATGAATAATTGCAAAAAAAAAAAAAAAAAACTCGAC
5' Read Nucleotide Sequence
>OriGene 5' read for NM_001924 unedited
TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCTGGGAGGCAGCG
GCCCAATTAGTGTCGTGCGGCCCGTGGCGAGGCGAGGTCCGGGGAGCGAGCGAGCAAGCA
AGGCGGGAGGGGTGGCCGGAGCTGCGGCGGCTGGCACAGGAGGAGGAGCCCGGGCGGGCG
AGGGGCGGCCGGAGAGCGCCAGGGCCTGAGCTGCCGGAGCGGCGCCTGTGAGTGAGTGCA
GAAAGCAGGCGCCCGCGCGCTAGCCGTGGCAGGAGCAGCCCGCACGCCGCGCTCTCTCCC
TGGGCGACCTGCAGTTTGCAATATGACTTTGGAGGAATTCTCGGCTGGAGAGCACAAGAC
CGAAAGGATGGATAAGGTGGGGGATGCCCTGGAGGAAGTGCTCAGCAAAGCCCTGAGTCA
GCGCACGATCACTGTCGGGGTGTACGAAGCGGCCAAGCTGCTCAACGTCGACCCCGATAA
CGTGGTGTTGTGCCTGCTGGCGGCGGACGAGGACGACGACAGAGATGTGGCTCTGCAGAT
CCACTTCACCCTGATCCAGGCGTTTTGCTGCGAGAACGACCATAAACATCCTGCGCGTCA
GCAACCCGGGGCCGGCTGGCGGAGCTCCTGCTCTAGAAGACCCGACGCTGGCCCCCGCGG
CGAGCGAAGGGCGCCGAGCAGCCCCCGGACCTGCCCTGCGTGCTGGTGACGAATCCCCAT
TTCTCCTCATGGAAGGGATCCTCGCCTTAGTGCACCTTATTTGTTTTTTGCCGGGAAAGT
TCGTTCCATGGACAATTGGGGCCCCAGGGATTATTCNTCCCTGGACCGGGGAGGCCATTT
GGAAGGAAAATACCTGACCCACATTGCCCGGAGGTTTTTGGAAAACCTTTGGAAGTTACT
TAACCCGTTCCTCCCTTCCCTGTAGGCAAGGATAACAGTAACC
3' Read Nucleotide Sequence
>OriGene 3' read for NM_001924 unedited
TCCGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTGCAATTATTCATACCAG
TTTATTGTAGGTATTGTGTTTCAAAAAATTATAGCATAAATAACTTTTCATGTCATAAAA
TAACTTAGTAGAATTTATAATAAAACTTTGAGAATTTAATATCTCATCAAAATACAATAA
AATATGGTTTTCAAATATGATTAACCCTTTCGGCTTTTCTTTTACTTGAGGGCATATAAA
CCAAAAATACCCAAACTATGGCTGCACACTTAAACTTTAACATATTTGGTTTATTTTAAT
GTAACTCAACCATTCTAAATTAATACATAGTTTTCCTTCCTGCATGGTTCTTTGTAATTT
TTGTTCCTTTTGACTTATTTTTCTGTTTCAACACAGCTTCCTTCTTCATTTTCACCTCTT
TCCATCTGCAAAGTCATCTATCTCCGGGCCCCCAGAACATGTAGTTGAACTCACTCAGCC
CCTTGGCATCAGTTTCTGTAATCCTTGCATCAGTGTAGGGAGTAACTGCTTGAGTAACTA
CAAAGGTATTTCAAAAACTTCAGTGCAATTTGGCTCAGTTATTTTCATTCAGATGCCATC
ACCGTTCAGGGAGATTAATCACTGGAACCCATTGATCCATGTAGCGACTTTCCCGGCAAA
AACCAATAAGTTGACTTAAGGCAGGATCCTTTCATTGAGATGAATGTGGATTCGTCACCA
GCACGCAATGCAGGTCCCGGGGGCTGCTTCGCCGCCTCGCTCGNCGCGGGGTCAGCGGTG
GTCTCCAAGACAAGAGCTCCGCCGCCGGCCCGGTTGCTGACGCGCAGAAGTTCATGCCCT
TTTGCACAAAAGCCCGGATAAGGTGAGTGGTATTGCAAACCACATTTGTTGCGGCCTTGT
CGCCGCCGCAGGACACACCCCGTATTGGGGCCACTTTAGCACTTGCCCGTTCGTCCCCCG
AACGGGACGGCCGCCGACCAGGGTTTGCTGCCCTCCTN
Restriction Sites NotI-NotI |
ACCN NM_001924
Insert Size 1360 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001924.2, NP_001915.1
RefSeq Size 1355 bp
RefSeq ORF 498 bp
Locus ID 1647
UniProt ID P24522
Cytogenetics 1p31.3
Domains Ribosomal_L7Ae
Protein Families Druggable Genome, Stem cell - Pluripotency
Protein Pathways Cell cycle, MAPK signaling pathway, p53 signaling pathway
Summary This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The DNA damage-induced transcription of this gene is mediated by both p53-dependent and -independent mechanisms. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.provided by RefSeq, Dec 2010
Transcript Variant: This variant (1) encodes the longest isoform (1).
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Documents
Resources
"NM_001924" in other vectors (6)
SKU Description Size Price
RC204005 GADD45A (Myc-DDK-tagged)-Human growth arrest and DNA-damage-inducible, alpha (GADD45A), transcript variant 1 10 ug
$225.00
RC204005L1 Lenti ORF clone of Human growth arrest and DNA-damage-inducible, alpha (GADD45A), transcript variant 1, Myc-DDK-tagged 10 ug
$525.00
RC204005L2 Lenti ORF clone of Human growth arrest and DNA-damage-inducible, alpha (GADD45A), transcript variant 1, mGFP tagged 10 ug
$525.00
RC204005L3 Lenti ORF clone of Human growth arrest and DNA-damage-inducible, alpha (GADD45A), transcript variant 1, Myc-DDK-tagged 10 ug
$525.00
RC204005L4 Lenti ORF clone of Human growth arrest and DNA-damage-inducible, alpha (GADD45A), transcript variant 1, mGFP tagged 10 ug
$525.00
RG204005 GADD45A (tGFP-tagged) - Human growth arrest and DNA-damage-inducible, alpha (GADD45A), transcript variant 1 10 ug
$425.00

file-alt Citations

CArG-driven GADD45α activated by resveratrol inhibits lung cancer cells
Shi, Geldenhuys, Sutariya et al
Genes Cancer (2015) 6 (5-6), 220-30
Snippet: Construction of suicide gene therapy vector. GADD45α open reading frame (ORF) was PCR amplified from GADD45α cDNA (Origene, MD) using specific primers: F- 5′-GGATCCGCCACCATGACTTTGGAGGAATTCTCG-3′. ... Cite SaveSaving
FigureFigureFigure
p53 controls hepatitis C virus non-structural protein 5A-mediated downregulation of GADD45α expression via the NF-κB and PI3K-Akt pathways
Cheng, Zhao, Zhang et al
J. Gen. Virol. (2013) 94 (Pt 2), 326-35 DOI: 10.1099/vir.0.046052-0
Cited by 28
AI Summary: OriGene’s GADD45α plasmid was used in this study to investigate the role of GADD45α in hepatitis C virus NS5A-mediated cellular effects, enabling the researchers to assess how ectopic expression of GADD45α impacts cell proliferation and gene regulation.
Snippet: expressing (vector-Huh7) cells were confirmed by PCR, Western blotting and sequencing. The GADD45α plasmid was purchased from OriGene. Reagents. Cells were incubated with NF-κB activation inhibitor QNZ (5 µM) and
FigureFigureFigure
Characterization of an alternatively spliced GADD45alpha, GADD45alpha1 isoform, in arsenic-treated epithelial cells
Zhang, Beezhold, Castranova et al
Mol Carcinog (2009) 48 (5), 454-64 DOI: 10.1002/mc.20483
Cited by 12
AI Summary: OriGene products contributed to this study by providing the GADD45alpha cDNA clone and related expression vectors, which were used for overexpression experiments to characterize the functional differences between the full-length GADD45alpha and the alternatively spliced GADD45alpha1 isoform.
FigureFigure
Methylation-Mediated Repression of GADD45 in Prostate Cancer and Its Role as a Potential Therapeutic Target
Ramachandran, Gopisetty, Gordian et al
Cancer Res (2009)
AI Summary: OriGene products, specifically the GADD45A cDNA clone and anti-GADD45A antibody, were utilized in this study to facilitate the overexpression and detection of GADD45A protein, enabling the researchers to investigate its role in methylation-mediated repression in prostate cancer.
Showing 1-4 of 4 papers.
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