EGR1 (NM_001964) Human Over-expression Lysate
SKU
LY419625
Transient overexpression lysate of early growth response 1 (EGR1) (as WB positive control)
$436.00
4 Weeks*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC209956 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_001964, NP_001955 |
| Synonyms | AT225; G0S30; KROX-24; NGFI-A; TIS8; ZIF-268; ZNF225 |
| Predicted MW | 57.3 kDa |
| Components | 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer |
| Storage | The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. |
| Shipping | Dry Ice |
| Bioactivity | DNA-binding activity |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). |
| Reference Data | |
| RefSeq | NP_001955 |
| Locus ID | 1958 |
| Cytogenetics | 5q31.2 |
| Protein Families | WB |
| Protein Pathways | Prion diseases |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Citations
Transcriptional Regulation of GDF15 by EGR1 Promotes Head and Neck Cancer Progression through a Positive Feedback Loop
Jin, Jung, Lim et al
Int J Mol Sci (2021) 22 (20) DOI: 10.3390/ijms222011151
Jin, Jung, Lim et al
Int J Mol Sci (2021) 22 (20) DOI: 10.3390/ijms222011151
Cited by 24
AI Summary: OriGene products, specifically the HA-GDF15 and EGR1 overexpression vectors, were used in this study to investigate the regulatory relationship and functional roles of GDF15 and EGR1 in head and neck cancer progression.
Snippet: The overexpression vector for HA-GDF15 and EGR1 was obtained from OriGene Technologies (Rockville, MD,
Epigenetic Modulation of Radiation-Induced Diacylglycerol Kinase Alpha Expression Prevents Pro-Fibrotic Fibroblast Response
Liu, Toth, Bakr et al
Cancers (Basel) (2021) 13 (10) DOI: 10.3390/cancers13102455
Liu, Toth, Bakr et al
Cancers (Basel) (2021) 13 (10) DOI: 10.3390/cancers13102455
Cited by 11
AI Summary: OriGene products contributed to this study by providing the Human DGKA cDNA clone (RC204307) and EGR1 cDNA clone (RC217125), which were used for overexpression experiments to investigate the roles of DGKA and EGR1 in radiation-induced pro-fibrotic responses in fibroblasts.
Showing 1-2 of 2 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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