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EGR1 (NM_001964) Human Over-expression Lysate

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SKU
LC419625
Lyophilized EGR1 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC209956
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001964, NP_001955
Synonyms AT225; G0S30; KROX-24; NGFI-A; TIS8; ZIF-268; ZNF225
Predicted MW 57.3 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_001955
Locus ID 1958
Cytogenetics 5q31.2
Protein Families WB
Protein Pathways Prion diseases
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Other "EGR1" proteins (2)
SKU Description Size Price
LY419625 Transient overexpression lysate of early growth response 1 (EGR1) (as WB positive control) 100 ug
$436.00
TP720992 Purified recombinant protein of Human early growth response 1 (EGR1) 10 ug
$340.00

file-alt Citations

Transcriptional Regulation of GDF15 by EGR1 Promotes Head and Neck Cancer Progression through a Positive Feedback Loop
Jin, Jung, Lim et al
Int J Mol Sci (2021) 22 (20) DOI: 10.3390/ijms222011151
Cited by 24
AI Summary: OriGene products, specifically the HA-GDF15 and EGR1 overexpression vectors, were used in this study to investigate the regulatory relationship and functional roles of GDF15 and EGR1 in head and neck cancer progression.
Snippet: The overexpression vector for HA-GDF15 and EGR1 was obtained from OriGene Technologies (Rockville, MD,
FigureFigureFigure
Epigenetic Modulation of Radiation-Induced Diacylglycerol Kinase Alpha Expression Prevents Pro-Fibrotic Fibroblast Response
Liu, Toth, Bakr et al
Cancers (Basel) (2021) 13 (10) DOI: 10.3390/cancers13102455
Cited by 11
AI Summary: OriGene products contributed to this study by providing the Human DGKA cDNA clone (RC204307) and EGR1 cDNA clone (RC217125), which were used for overexpression experiments to investigate the roles of DGKA and EGR1 in radiation-induced pro-fibrotic responses in fibroblasts.
FigureFigureFigure
Showing 1-2 of 2 papers.
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