OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

EIF5A (NM_001970) Human Over-expression Lysate

Be the first to review this product
SKU
LY419616
Transient overexpression lysate of eukaryotic translation initiation factor 5A (EIF5A), transcript variant B (as WB positive control)
  $436.00
In Stock*
Bulk/Customize

Frequently Bought Together (3)

TA332499 Rabbit anti-EIF5A Polyclonal Antibody
100 ul
$410.00
TA811000S beta Actin Mouse Monoclonal Antibody, Clone OTI1, Loading Control 30 ul
$200.00
TA592569 DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
100 ul
$519.00

Other products for "EIF5A or NM_001970"

Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC200770
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001970, NP_001961
Synonyms eIF-4D; EIF-5A; EIF5A1; eIF5AI
Predicted MW 16.8 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_001961
Locus ID 1984
Cytogenetics 17p13.1
Protein Families WB
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
Other "EIF5A" proteins (11)
SKU Description Size Price
PH300770 EIF5A MS Standard C13 and N15-labeled recombinant protein (NP_001961) 10 ug
$3,360.00
PH326600 EIF5A MS Standard C13 and N15-labeled recombinant protein (NP_001137233) 10 ug
$3,360.00
PH326664 EIF5A MS Standard C13 and N15-labeled recombinant protein (NP_001137234) 10 ug
$3,360.00
LC419616 Lyophilized EIF5A HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC428326 Lyophilized EIF5A HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC428327 Lyophilized EIF5A HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY428326 Transient overexpression lysate of eukaryotic translation initiation factor 5A (EIF5A), transcript variant C (as WB positive control) 100 ug
$436.00
LY428327 Transient overexpression lysate of eukaryotic translation initiation factor 5A (EIF5A), transcript variant D (as WB positive control) 100 ug
$436.00
TP300770 Recombinant protein of human eukaryotic translation initiation factor 5A (EIF5A), transcript variant B, 20 µg 20 ug
$737.00
TP326600 Recombinant protein of human eukaryotic translation initiation factor 5A (EIF5A), transcript variant C, 20 µg 20 ug
$737.00
TP326664 Recombinant protein of human eukaryotic translation initiation factor 5A (EIF5A), transcript variant D, 20 µg 20 ug
$737.00

file-alt Citations

Bruton tyrosine kinase inhibitors preserve anti-CD19 chimeric antigen receptor T-cell functionality and reprogram tumor micro-environment in B-cell lymphoma
Luo, Li, Wu et al
Cytotherapy (2023) 25 (7), 739-749 DOI: 10.1016/j.jcyt.2023.03.005
Cited by 29
AI Summary: In this study, OriGene products—including the Human CD19 (NM_001770) cDNA clone (RC208447) and Human GAPDH (NM_002046) qPCR Primer Pair (HPH000001)—were used to assess gene expression and validate experimental findings related to the effects of Bruton tyrosine kinase inhibitors on CAR T-cell function and the tumor microenvironment in B-cell lymphoma.
Showing 1-1 of 1 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products