IRF1 (NM_002198) Human Over-expression Lysate
SKU
LY419474
Transient overexpression lysate of interferon regulatory factor 1 (IRF1) (as WB positive control)
$436.00
In Stock*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC203500 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_002198, NP_002189 |
| Synonyms | IRF-1; MAR |
| Predicted MW | 36.5 kDa |
| Components | 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer |
| Storage | The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. |
| Shipping | Dry Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). |
| Reference Data | |
| RefSeq | NP_002189 |
| Locus ID | 3659 |
| Cytogenetics | 5q31.1 |
| Protein Families | WB |
Reviews
Documents
| Product Manuals |
| FAQs |
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Resources
| Recombinant Protein Resources |
Other "IRF1" proteins (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH303500 | IRF1 MS Standard C13 and N15-labeled recombinant protein (NP_002189) | 10 ug |
$3,360.00
|
|
| LC419474 | Lyophilized IRF1 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| TP303500 | Recombinant protein of human interferon regulatory factor 1 (IRF1), 20 µg | 20 ug |
$867.00
|
|
Citations
Interferon regulatory factor 1-Rab27a regulated extracellular vesicles promote liver ischemia/reperfusion injury
Yang, Du, Goswami et al
Hepatology (2017)
Yang, Du, Goswami et al
Hepatology (2017)
AI Summary: OriGene provided the human IRF-1 expression plasmid and backbone plasmid, which were used in this study to investigate the regulatory role of IRF-1 in Rab27a expression and extracellular vesicle secretion during liver ischemia/reperfusion injury.
Snippet: purchased from American Type Culture Collection (ATCC;Rockville, MD, USA). Human IRF-1 expression plasmid and backbone plasmid were ordered from Origene (Rockville, MD). β-galactosidase expression (β-Gal) plasmid was purchased from Promega (Madison
Immune mediators regulate CFTR expression through a bifunctional airway-selective enhancer
Zhang, Leir, Harris
Mol. Cell. Biol. (2013) 33 (15), 2843-53 DOI: 10.1128/MCB.00003-13
Zhang, Leir, Harris
Mol. Cell. Biol. (2013) 33 (15), 2843-53 DOI: 10.1128/MCB.00003-13
Cited by 1
Showing 1-2 of 2 papers.
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